PdNF-YB21 positively regulated root lignin structure in poplar
PdNF-YB21 positively regulated root lignin structure in poplar
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PdNF-YB21正向调节杨树根木质素结构
DOI：
10.1016/j.indcrop.2021.113609
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发表时间：
2021-09
影响因子：
5.9
通讯作者：
Xia Xinli
中科院分区：
文献类型：
--
作者：
Zhang Yue;Lin Shiwei;Zhou Yangyan;Wen Jialong;Kang Xihui;Han Xiao;Liu Chao;Yin Weilun;Xia Xinli
白杨是世界上重要的生物质资源和生产木质纤维素生物燃料的优势树种。今天，杨树的使用主要集中在地上部分。但作为树木的重要组成部分，白杨根系发达，根系约占树木体积的20%，根系物质体积十分可观，利用潜力巨大。根木质素是白杨生物量生产的核心。但对根系木质素结构的研究却很少。最近，我们发现一个根特异性转录因子，核因子Y亚基B21（NF-YB 21），参与调控根的生长和木质化。本研究采用生理学、转基因、转录组学、代谢组学、共聚焦拉曼显微镜（CRM）和核磁共振（NMR）等技术，对PdNF-YB 21对白杨根系木质素结构的调控机制进行了深入研究。转录组和代谢组分析结果表明，转基因植株中存在更多与S-木质素合成相关的基因和代谢产物。根系硬度测定、CRM和NMR结果表明，过量表达PdNF-YB 21显著提高了根系硬度，增加了根系木质素含量和S/G比值，并增加了根系中葡聚糖、木聚糖和木质素的含量，从而促进了根系的生长。总之，本研究不仅阐明了PdNF-YB 21对白杨根系木质素结构的调控机制，而且为当前生物炼制过程中白杨新品种的选育提供了指导。
Poplar is a key player in biomass resource and a dominant species for lignocellulosic biofuel production around the world. Today, the use of poplars is mainly concentrated in the aboveground parts. However, as an important part of trees, the poplar root system developed, roots account for about 20 % of the volume of the tree, and the root material volume is very considerable and its utilization potential is huge. The root lignin is central to biomass production in poplar. However, there are very few studies on root lignin structure. Recently, we found a root-specific transcription factor, NUCLEAR FACTOR Y subunit B21 (NF-YB21), is involved in regulating root growth and lignification. In the present study, physiology, transgenosis, transcriptomics, metabolome, Confocal Raman Microscopy (CRM) and Nuclear Magnetic Resonance (NMR) techniques were performed to further investigate the regulatory mechanisms of PdNF-YB21 on lignin structure in poplar roots. The results of transcriptome and metabolome showed that there were more genes and metabolites related to S-lignin synthesis in transgenic plants. Root firmness measurements, CRM and NMR results showed that overexpression ofPdNF-YB21dramatically improved root hardness, increased root lignin content and S/G ratio, and increased the concentrations of glucan, xylan and lignin in roots, which promoted root growth. In short, this present study not only illustrated the regulatory mechanisms ofPdNF-YB21on lignin structure in poplar roots, but also proposed a directive breeding of new poplar species for current biorefinery process.
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影响因子：
4.8
作者：
Jing-hua Sun;Jing-jing Luo;L. Tian;Chun-li Li;Y. Xing;Yuan-Yue Shen
通讯作者：
Jing-hua Sun;Jing-jing Luo;L. Tian;Chun-li Li;Y. Xing;Yuan-Yue Shen
影响因子：
8.4
作者：
Hanmin Wang;Cheng-Ye Ma;Hanyin Li;Tianying Chen;J. Wen;Xuefei Cao;Xiluan Wang;T. Yuan;R. Sun
通讯作者：
Hanmin Wang;Cheng-Ye Ma;Hanyin Li;Tianying Chen;J. Wen;Xuefei Cao;Xiluan Wang;T. Yuan;R. Sun
影响因子：
6.3
作者：
Zhang T;Wyman CE;Jakob K;Yang B
通讯作者：
Yang B
影响因子：
7
作者：
Cajuste, Jacques F.;Lafuente, Maria T.
通讯作者：
Lafuente, Maria T.
影响因子：
3.7
作者：
K. Yugi;Shinya Kuroda
通讯作者：
K. Yugi;Shinya Kuroda