HrrF is the Fur-regulated small RNA in nontypeable Haemophilus influenzae.
HrrF is the Fur-regulated small RNA in nontypeable Haemophilus influenzae.
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DOI：
10.1371/journal.pone.0105644
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Munson RS Jr
中科院分区：
文献类型：
--
作者：
Santana EA;Harrison A;Zhang X;Baker BD;Kelly BJ;White P;Liu Y;Munson RS Jr
不可分型的流感嗜血杆菌（NTHi）是存在于鼻咽部的革兰氏阴性细菌。NTHi还可引起多种上呼吸道和下呼吸道疾病，包括鼻窦炎、结膜炎、支气管炎和中耳炎。在许多细菌物种中，铁摄取调节剂（Fur）通过负调节铁摄取系统的表达而充当铁稳态的全局调节剂。然而，在NTHi菌株86- 028 NP和许多其他细菌物种中，存在多个实例，其中Fur对基因表达产生积极影响。已知许多Fur正调控的实例通过小RNA中间体间接发生。然而，在NTHi中没有描述小RNA的实例。因此，我们使用RNA-Seq分析来分析NTHi菌株86- 028 NPrpsL和同基因86- 028 NPrpsL Δfur菌株的转录组，以鉴定Fur调节的基因间转录物。从这个分析中，我们确定了hrrF，第一个小RNA描述的任何嗜血杆菌物种。这种小RNA的直向同源物仅存在于其他巴斯德菌科中。我们的分析表明，当铁水平低时，HRRF表达最大。此外，显示Fur结合hrrF启动子的上游。RNA-Seq分析用于鉴定HRRF的靶标，所述靶标包括其产物参与DNA摄取、脱氧核糖核苷酸合成和氨基酸生物合成的基因。HRRF的稳定性不依赖于RNA分子伴侣Hfq。这项研究是研究小RNA在NTHi中改变基因表达以响应铁限制的作用的第一步。
Nontypeable Haemophilus influenzae (NTHi) are Gram-negative commensal bacteria that reside in the nasopharynx. NTHi can also cause multiple upper and lower respiratory tract diseases that include sinusitis, conjunctivitis, bronchitis, and otitis media. In numerous bacterial species the ferric uptake regulator (Fur) acts as a global regulator of iron homeostasis by negatively regulating the expression of iron uptake systems. However in NTHi strain 86-028NP and numerous other bacterial species there are multiple instances where Fur positively affects gene expression. It is known that many instances of positive regulation by Fur occur indirectly through a small RNA intermediate. However, no examples of small RNAs have been described in NTHi. Therefore we used RNA-Seq analysis to analyze the transcriptome of NTHi strain 86-028NPrpsL and an isogenic 86-028NPrpsLΔfur strain to identify Fur-regulated intergenic transcripts. From this analysis we identified HrrF, the first small RNA described in any Haemophilus species. Orthologues of this small RNA exist only among other Pasteurellaceae. Our analysis showed that HrrF is maximally expressed when iron levels are low. Additionally, Fur was shown to bind upstream of the hrrF promoter. RNA-Seq analysis was used to identify targets of HrrF which include genes whose products are involved in molybdate uptake, deoxyribonucleotide synthesis, and amino acid biosynthesis. The stability of HrrF is not dependent on the RNA chaperone Hfq. This study is the first step in an effort to investigate the role small RNAs play in altering gene expression in response to iron limitation in NTHi.
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影响因子：
3.7
作者：
Amarasinghe, J. J.;Connell, T. D.;Haase, E. M.
通讯作者：
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影响因子：
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作者：
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