Biopolymer Patterning-Directed Secretion in Mucoid and Nonmucoid Strains of Pseudomonas aeruginosa Revealed by Multimodal Chemical Imaging.
Biopolymer Patterning-Directed Secretion in Mucoid and Nonmucoid Strains of Pseudomonas aeruginosa Revealed by Multimodal Chemical Imaging.
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多模式化学成像揭示铜绿假单胞菌粘液和非粘液菌株中生物聚合物模式导向分泌。
DOI：
10.1021/acsinfecdis.0c00765
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发表时间：
2021-03-12
影响因子：
5.3
通讯作者：
Bohn PW
中科院分区：
文献类型：
--
作者：
Jia J;Ellis JF;Cao T;Fu K;Morales-Soto N;Shrout JD;Sweedler JV;Bohn PW
喹诺酮，绿脓菌素，和鼠李糖脂的生产研究铜绿假单胞菌的空间模式粘蛋白-一种糖蛋白的重要感染肺上皮细胞。将质谱成像和共聚焦拉曼显微术结合起来，以探测来自粘液和非粘液菌株的铜绿假单胞菌生物膜，所述铜绿假单胞菌生物膜生长在平版印刷定义的图案上。比较了来自图案化与未图案化以及粘蛋白与巯基十一烷酸（MUA）表面上的生物膜的喹诺酮签名。微生物附着伴随着2-烷基-4-喹诺酮以及来自粘液和非粘液菌株的鼠李糖脂的分泌。绿脓菌素也被检测到的生物膜和上清液中的粘液样菌株。分泌因子的时空分布的显着差异，观察菌株之间和不同的表面图案化条件。类粘蛋白菌株对组成和图案化敏感，而非类粘蛋白则不敏感，并且在促进类粘蛋白菌株中的群落发育方面，非图案化表面优于图案化表面，并且粘蛋白优于MUA。此外，粘液菌株分泌的毒力因子绿脓菌素的方式，与痛苦。两种鼠李糖脂的相对丰度的变化，观察到在粘液菌株暴露于粘蛋白，而在非粘液菌株中观察到最小的变化。粘液和非粘液菌株之间的差异与它们的菌株特异性物候相一致，其中粘液菌株形成高度保护和撤回的生物膜，其在有限的条件下实现PQS生产。基于拉曼和质谱的化学成像被用来阐明底物图案化引起的信号分子的空间和时间分布。铜绿假单胞菌的粘液和非粘液菌株对表面图案化和糖蛋白粘蛋白的存在有不同的反应。
Quinolone, pyocyanin, and rhamnolipid production are studied in Pseudomonas aeruginosa by spatially patterning mucin — a glycoprotein important to infection of lung epithelia. Mass spectrometric imaging and confocal Raman microscopy are combined to probe P. aeruginosa biofilms from mucoid and non-mucoid strains, grown on lithographically-defined patterns. Quinolone signatures from biofilms on patterned vs. unpatterned and mucin vs. mercaptoundecanoic acid, MUA, surfaces are compared. Microbial attachment is accompanied by secretion of 2-alkyl-4-quinolones as well as rhamnolipids from the mucoid and non-mucoid strains. Pyocyanin is also detected both in the biofilm and in the supernatant in the mucoid strain only. Significant differences in the spatiotemporal distributions of secreted factors are observed between strains and among different surface patterning conditions. The mucoid strain is sensitive to composition and patterning while non-mucoid is not, and in promoting community development in the mucoid strain, non-patterned surfaces are better than patterned, and mucin is better than MUA. Also, the mucoid strain secretes the virulence factor pyocyanin in a way that correlates with distress. A change in the relative abundance for two rhamnolipids is observed in the mucoid strain during exposure to mucin, whereas minimal variation is observed in the non-mucoid strain. Differences between mucoid and non-mucoid strains are consistent with their strain-specific phenology, in which the mucoid strain develops highly protected and withdrawn biofilms that achieve PQS production under limited conditions. Raman- and mass spectrometry-based chemical imaging are used to elucidate the spatial and temporal distributions of signaling molecules elicited by substrate patterning. Mucoid and non-mucoid strains of Pseudomonas aeruginosa respond differently to surface patterning and the presence of the glycoprotein, mucin.
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影响因子：
3
作者：
Gaifulina R;Maher AT;Kendall C;Nelson J;Rodriguez-Justo M;Lau K;Thomas GM
通讯作者：
Thomas GM
抗药性金黄色葡萄球菌菌株与拉曼微光谱法揭示了不同的生化特征。
影响因子：
5.3
作者：
Ayala OD;Wakeman CA;Pence IJ;Gaddy JA;Slaughter JC;Skaar EP;Mahadevan-Jansen A
通讯作者：
Mahadevan-Jansen A
影响因子：
11.3
作者：
Heeb S;Fletcher MP;Chhabra SR;Diggle SP;Williams P;Cámara M
通讯作者：
Cámara M
DOI：
10.1099/00221287-13-3-572
发表时间：
1955-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
HOLLOWAY, BW
通讯作者：
HOLLOWAY, BW
MSIREADER v1.0：为目标和非目标分析而发展的开源质谱成像软件。
DOI：
10.1007/s13361-017-1809-6
发表时间：
2018-01
影响因子：
3.2
作者：
Bokhart MT;Nazari M;Garrard KP;Muddiman DC
通讯作者：
Muddiman DC