Effects of different starch sources on Bacillus spp. in intestinal tract and expression of intestinal development related genes of weanling piglets.
Effects of different starch sources on Bacillus spp. in intestinal tract and expression of intestinal development related genes of weanling piglets.
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DOI：
10.1007/s11033-011-0932-x
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发表时间：
2012-02
影响因子：
2.8
通讯作者：
Huang ZQ
中科院分区：
文献类型：
--
作者：
Han GQ;Xiang ZT;Yu B;Chen DW;Qi HW;Mao XB;Chen H;Mao Q;Huang ZQ
本试验旨在研究不同淀粉源对断奶仔猪肠道芽孢杆菌及肠道发育相关基因表达的影响。28头PIC公仔猪按初始体重（出生胎次相近，断奶时间21±1.5 d）均匀分为4组。4个处理的饲粮分别为玉米淀粉、小麦淀粉、木薯淀粉和豌豆淀粉，直链淀粉与支链淀粉的比值分别为0.21、0.24、0.12和0.52。采用实时定量聚合酶链反应技术：(1)根据GenBank上芽孢杆菌最大种类的16S rRNA序列设计的引物和探针，检测芽孢杆菌的基因组DNA，定量测定仔猪肠道食糜中芽孢杆菌的数量；(2)测定十二指肠、空肠和回肠胰高血糖素样肽2 （GLP-2）、胰岛素样生长因子1 （IGF-1）和表皮生长因子（EGF） mRNA水平。结果表明，饲喂豌豆淀粉的仔猪全肠内容物中芽孢杆菌数量和全菌百分比最高（P < 0.05）。玉米淀粉组与小麦淀粉组仔猪全肠道所有细菌和芽孢杆菌拷贝数差异不显著（P < 0.05）。此外，高直链/支链淀粉比豌豆淀粉处理空肠和回肠GLP-2、IGF-1 mRNA表达量显著高于其他3种处理（P < 0.05），而木薯淀粉处理显著降低其mRNA表达量（P < 0.05）。各组EGF mRNA水平差异无统计学意义。本研究发现，高直链淀粉/支链淀粉比例的淀粉显著提高了肠道各节段芽孢杆菌的数量和肠道发育相关基因的mRNA水平。
The study was conducted to evaluate the effects of different starch sources on Bacillus spp. in intestinal tract and expression of intestinal development related genes of weanling piglets. Twenty-eight PIC male piglets were divided into four homogeneous groups according to initial body weight (similar birth and parity, weaned at 21 ± 1.5 days). Diets for the four treatments consisted of corn starch, wheat starch, tapioca starch and pea starch with the determined ratio for amylose to amylopectin of 0.21, 0.24, 0.12 and 0.52 respectively. Real-time quantitative polymerase chain reaction was applied to: (1) detect genomic DNA of Bacillus and to quantify the number of Bacillus in the intestinal tract chyme of piglets with the primers and probe which designed based on the 16S rRNA sequences of maximum species of Bacillus on GenBank; (2) measure the mRNA level of glucagon-like peptide 2 (GLP-2), insulin-like growth factors 1 (IGF-1) and epidermal growth factor (EGF) in duodenum, jejunum and ileum. Results showed that the number of Baciilus and the percentage based on all bacteria in the whole intestinal content of piglets fed pea starch was highest in all groups (P < 0.05). There was no significant differance on copy numbers for all bacteria and Bacillus in the whole intestinal tract of piglets between the corn starch group and wheat starch group (P > 0.05). In addition, the expression level of GLP-2, IGF-1 mRNA in jejunum and ileum of pea starch treatment (the high amylose/amylopectin ratio) were increased while the tapioca starch decreased their mRNA level significantly compared to other three treatments (P < 0.05). There was no significant difference for the mRNA level of EGF in each group. The present study revealed that high amylose/amylopectin ratio of starches significantly enhanced the numbers of Bacillus in all segments of intestine and the mRNA level of intestinal development related genes.
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影响因子：
3.5
作者：
Erkens T;Van Poucke M;Vandesompele J;Goossens K;Van Zeveren A;Peelman LJ
通讯作者：
Peelman LJ
影响因子：
2.1
作者：
BILIADERIS, CG
通讯作者：
BILIADERIS, CG
影响因子：
4.4
作者：
De Medici, D;Croci, L;Toti, L
通讯作者：
Toti, L
影响因子：
4.3
作者：
Deng, Shu-Xuan;Cheng, An-Chun;Cao, Ping
通讯作者：
Cao, Ping
影响因子：
4.4
作者：
Kaplan, H;Hutkins, RW
通讯作者：
Hutkins, RW