Forespore Targeting of SpoVD in Bacillus subtilis Is Mediated by the N-Terminal Part of the Protein
Forespore Targeting of SpoVD in Bacillus subtilis Is Mediated by the N-Terminal Part of the Protein
复制标题
枯草芽孢杆菌中 SpoVD 的前孢子靶向是由蛋白质 N 端部分介导的
DOI：
10.1128/jb.00163-18
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发表时间：
2018
影响因子：
3.2
通讯作者：
Ewa Bukowska
中科院分区：
文献类型：
--
作者：
Margareth Sidarta;Dongdong Li;L. Hederstedt;Ewa Bukowska
摘要SpoVD和PBP4b是结构上非常相似的高分子量B类青霉素结合蛋白，在枯草芽孢杆菌的孢子形成早期产生。已知SpoVD对于内孢子皮层的合成和耐热孢子的产生是必不可少的。PBP4b的作用仍然是个谜。这两种蛋白质都是在母细胞的细胞质中合成的。PBP4b留在母细胞的细胞膜上，而SpoVD则聚集在前孔外膜上。通过使用SpoVD/PBP4b嵌合体和互换的蛋白质结构域，我们证明了SpoVD的N端部分，包含单一的跨膜区，决定了蛋白质的前孔靶向。重要性β-内酰胺类抗生素的靶标是青霉素结合蛋白(PBPs)，它在细胞壁肽聚糖的合成中发挥作用。包括芽孢杆菌和梭状芽孢杆菌在内的一些属的细菌可以形成内孢子。内孢子对严酷的物理化学条件的极端抵抗力是临床微生物学和食品工业关注的问题。内孢子皮质层生物发生是研究肽聚糖合成的实验模型系统。营养细菌细胞向内孢子的分化包括在产孢子细胞的细胞质内形成前孔。一些蛋白质，包括一些多溴联苯，在前孔中积累。例如，对细菌细胞中这种亚细胞靶向蛋白质背后的分子机制的了解可以导致一种阻止孢子形成过程的方法。
ABSTRACT SpoVD and PBP4b are structurally very similar high-molecular-weight, class B penicillin-binding proteins produced early during sporulation in Bacillus subtilis. SpoVD is known to be essential for endospore cortex synthesis and thereby the production of heat-resistant spores. The role of PBP4b is still enigmatic. Both proteins are synthesized in the cytoplasm of the mother cell. PBP4b remains in the cytoplasmic membrane of the mother cell, whereas SpoVD accumulates in the forespore outer membrane. By the use of SpoVD/PBP4b chimeras with swapped protein domains, we show that the N-terminal part of SpoVD, containing the single transmembrane region, determines the forespore targeting of the protein. IMPORTANCE Beta-lactam-type antibiotics target penicillin-binding proteins (PBPs), which function in cell wall peptidoglycan synthesis. Bacteria of a subset of genera, including Bacillus and Clostridium species, can form endospores. The extreme resistance of endospores against harsh physicochemical conditions is of concern in clinical microbiology and the food industry. Endospore cortex layer biogenesis constitutes an experimental model system for research on peptidoglycan synthesis. The differentiation of a vegetative bacterial cell into an endospore involves the formation of a forespore within the cytoplasm of the sporulating cell. A number of proteins, including some PBPs, accumulate in the forespore. An understanding of the molecular mechanisms behind such subcellular targeting of proteins in bacterial cells can, for example, lead to a means of blocking the process of sporulation.
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影响因子：
11.3
作者：
Higgins D;Dworkin J
通讯作者：
Dworkin J
影响因子：
3.7
作者：
Dworkin, Jonathan
通讯作者：
Dworkin, Jonathan
影响因子：
5.6
作者：
Wang, ST;Setlow, B;Eichenberger, P
通讯作者：
Eichenberger, P
影响因子：
3.3
作者：
Tan IS;Ramamurthi KS
通讯作者：
Ramamurthi KS