Organized nanofibrous scaffolds that mimic the macroscopic and microscopic architecture of the knee meniscus.
Organized nanofibrous scaffolds that mimic the macroscopic and microscopic architecture of the knee meniscus.
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DOI：
10.1016/j.actbio.2012.10.018
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发表时间：
2013-01
影响因子：
9.7
通讯作者：
Mauck, Robert L.
中科院分区：
文献类型：
--
作者：
Fisher, Matthew B.;Henning, Elizabeth A.;Soeegaard, Nicole;Esterhai, John L.;Mauck, Robert L.
半月板是月牙形的纤维软骨组织，其结构组织由局部排列的致密胶原束组成，但在宏观方向性上呈现连续变化。这种圆周模式对于膝关节的负荷传递是必要的，并且是组织工程构建体的一个关键设计参数。为了解决这个问题，我们开发了一种新的静电纺丝方法来生产由圆周排列（CircAl）纳米纤维组成的支架，对其结构和力学性能进行了量化，并与传统的线性排列（LinAl）支架进行了比较。在CircAl支架中纤维是局部定向的，但它们的定向随位置变化很大（p<0.05）。LinAl纤维在类似的长度尺度上定向没有变化（p>0.05）。CircAl支架的细胞接种导致了类似的细胞方向性。对CircAl支架的力学分析显示支架长度和区域之间存在显著的相互作用（p<0.05），其中支架边缘附近的拉伸模量随着支架长度的增加而降低。在LinAl样本中未检测到差异（p>0.05）。纤维沉积过程的模拟产生了与实验观察到的纤维组织和力学性能相匹配的“理论”纤维群体。这些具有空间变化的局部定向和力学性能的新型支架将使功能性解剖半月板构建体的形成成为可能。
The menisci are crescent-shaped fibrocartilaginous tissues whose structural organization consists of dense collagen bundles that are locally aligned, but show a continuous change in macroscopic directionality. This circumferential patterning is necessary for load transmission across the knee joint and is a key design parameter for tissue engineered constructs. To address this issue, we developed a novel electrospinning method to produce scaffolds composed of circumferentially aligned (CircAl) nanofibers, quantified their structure and mechanics, and compared them to traditional linearly aligned (LinAl) scaffolds. Fibers were locally oriented in CircAl scaffolds, but their orientation varied considerably as a function of position (p<0.05). LinAl fibers did not change in orientation over a similar length scale (p>0.05). Cell seeding of CircAl scaffolds resulted in a similar cellular directionality. Mechanical analysis of CircAl scaffolds revealed significant interactions between scaffold length and region (p<0.05), where the tensile modulus near the edge of the scaffolds decreased with increasing scaffold length. No differences were detected in LinAl specimens (p>0.05). Simulation of the fiber deposition process produced “theoretical” fiber populations that matched the fiber organization and mechanical properties observed experimentally. These novel scaffolds, with spatially varying local orientation and mechanics, will enable the formation of functional anatomic meniscus constructs.
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影响因子：
14
作者：
D'Amore A;Stella JA;Wagner WR;Sacks MS
通讯作者：
Sacks MS
DOI：
10.1023/b:jmsm.0000021114.39595.1e
发表时间：
2004-04-01
影响因子：
3.7
作者：
Heijkants, RGJC;Van Calck, RV;Veth, RPH
通讯作者：
Veth, RPH
影响因子：
4.8
作者：
Kelly, Bryan T.;Robertson, William;Rodeo, Scott A.
通讯作者：
Rodeo, Scott A.
DOI：
10.1115/1.4002490
发表时间：
2010-10-01
影响因子：
1.7
作者：
Elsner, Jonathan J.;Portnoy, Sigal;Linder-Ganz, Eran
通讯作者：
Linder-Ganz, Eran
影响因子：
9.7
作者：
Li, Wan-Ju;Cooper, James A., Jr.;Tuan, Rocky S.
通讯作者：
Tuan, Rocky S.