Quantitative vibrational imaging by hyperspectral stimulated Raman scattering microscopy and multivariate curve resolution analysis.
Quantitative vibrational imaging by hyperspectral stimulated Raman scattering microscopy and multivariate curve resolution analysis.
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DOI：
10.1021/ac3019119
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发表时间：
2013-01-02
影响因子：
7.4
通讯作者：
Cheng, Ji-Xin
中科院分区：
文献类型：
--
作者：
Zhang, Delong;Wang, Ping;Slipchenko, Mikhail N.;Ben-Amotz, Dor;Weiner, Andrew M.;Cheng, Ji-Xin
光谱成像已经成为揭示生物环境中特定分子的一种越来越重要的方法。为了实现这一目标，我们展示了高光谱受激拉曼损耗（SRL）成像脉冲内光谱扫描通过飞秒脉冲整形器。通过多元曲线分辨率（MCR）方法进一步分析SRL图像的高光谱堆叠，以重建每个单独组分的定量浓度图像并检索相应的振动拉曼光谱。使用这些方法，我们展示了二甲基亚砜浓度在水溶液和脂肪组织中的定量映射。此外，对乳腺癌细胞的SRL图像进行MCR，以生成主化学成分沿着其各自的振动光谱的图。这些结果显示了高光谱SRL显微镜通过分辨重叠的拉曼谱带对复杂生物分子混合物进行定量成像的巨大能力和潜力。
Spectroscopic imaging has been an increasingly critical approach for unveiling specific molecules in biological environments. Towards this goal, we demonstrate hyperspectral stimulated Raman loss (SRL) imaging by intra-pulse spectral scanning through a femtosecond pulse shaper. The hyperspectral stack of SRL images is further analyzed by a multivariate curve resolution (MCR) method to reconstruct quantitative concentration images for each individual component and retrieve the corresponding vibrational Raman spectra. Using these methods, we demonstrate quantitative mapping of dimethyl sulfoxide concentration in aqueous solutions and in fat tissue. Moreover, MCR is performed on SRL images of breast cancer cells to generate maps of principal chemical components along with their respective vibrational spectra. These results show the great capability and potential of hyperspectral SRL microscopy for quantitative imaging of complicated biomolecule mixtures through resolving overlapped Raman bands.
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影响因子：
3.6
作者：
Jones, Gareth W.;Marks, Daniel L.;Boppart, Stephen A.
通讯作者：
Boppart, Stephen A.
影响因子：
7.4
作者：
Chowdary, Praveen D.;Benalcazar, Wladimir A.;Gruebele, Martin
通讯作者：
Gruebele, Martin
DOI：
10.1126/science.1198704
发表时间：
2011-05-06
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Bendall SC;Simonds EF;Qiu P;Amir el-AD;Krutzik PO;Finck R;Bruggner RV;Melamed R;Trejo A;Ornatsky OI;Balderas RS;Plevritis SK;Sachs K;Pe'er D;Tanner SD;Nolan GP
通讯作者：
Nolan GP
影响因子：
3.1
作者：
HORNBECK, LJ
通讯作者：
HORNBECK, LJ
DOI：
10.1016/j.chemolab.2004.12.007
发表时间：
2005-03-28
影响因子：
3.9
作者：
Jaumot, J;Gargallo, R;Tauler, R
通讯作者：
Tauler, R