Cardiolipin Regulates the Activity of the Mitochondrial ABC Transporter ABCB10.
Cardiolipin Regulates the Activity of the Mitochondrial ABC Transporter ABCB10.
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DOI：
10.1021/acs.biochem.3c00417
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发表时间：
2023-11-07
期刊：
影响因子：
2.9
通讯作者：
Laganowsky A
中科院分区：
文献类型：
--
作者：
Zhang T;Lyu J;Zhu Y;Laganowsky A
The ATP-binding cassette (ABC) transporter ABCB10 resides in the inner membrane of mitochondria and is implicated in erythropoiesis. Mitochondria from different cell types share some specific characteristics, one of which is the high abundance of cardiolipin. Although previous studies have provided insight into ABCB10, the affinity and selectivity of this transporter toward lipids, particularly those found in the mitochondria, remain poorly understood. Here, native mass spectrometry is used to directly monitor the binding events of lipids to human ABCB10. The results reveal that ABCB10 binds avidly to cardiolipin with an affinity significantly higher than that of other phospholipids. The first three binding events of cardiolipin display positive cooperativity, which is suggestive of specific cardiolipin-binding sites on ABCB10. Phosphatidic acid is the second-best binder of the lipids investigated. The bulk lipids, phosphatidylcholine and phosphatidylethanolamine, display the weakest binding affinity for ABCB10. Other lipids bind ABCB10 with a similar affinity. Functional assays show that cardiolipin regulates the ATPase activity of ABCB10 in a dose-dependent fashion. ATPase activity of ABCB10 was also impacted in the presence of other lipids but to a lesser extent than cardiolipin. Taken together, ABCB10 has a high binding affinity for cardiolipin, and this lipid also regulates the ATPase activity of the transporter.
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DOI：
10.1007/s13361-018-1976-0
发表时间：
2019-01
影响因子：
3.2
作者：
Poltash ML;McCabe JW;Patrick JW;Laganowsky A;Russell DH
通讯作者：
Russell DH
完整膜蛋白复合物的质谱法。
影响因子：
14.8
作者：
通讯作者：
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影响因子：
5.5
作者：
Gonzalez-Ibanez AM;Ruiz LM;Jensen E;Echeverria CA;Romero V;Stiles L;Shirihai OS;Elorza AA
通讯作者：
Elorza AA
影响因子：
16.6
作者：
Cong X;Liu Y;Liu W;Liang X;Laganowsky A
通讯作者：
Laganowsky A
影响因子：
15
作者：
Cong, Xiao;Liu, Yang;Laganowsky, Arthur
通讯作者：
Laganowsky, Arthur