The Potential Role of PKA/CREB Signaling Pathway Concerned with Gastrodin Administration on Methamphetamine-Induced Conditioned Place Preference Rats and SH-SY5Y Cell Line
The Potential Role of PKA/CREB Signaling Pathway Concerned with Gastrodin Administration on Methamphetamine-Induced Conditioned Place Preference Rats and SH-SY5Y Cell Line
复制标题
天麻素给药相关的 PKA/CREB 信号通路对甲基苯丙胺诱导的条件位置偏好大鼠和 SH-SY5Y 细胞系的潜在作用
DOI：
10.1007/s12640-019-00150-7
复制
发表时间：
2020-01
影响因子：
3.7
通讯作者：
Li-Hua Li
中科院分区：
文献类型：
--
作者：
Gen-Meng Yang;Lu Li;Feng-Lin Xue;Chen-Li Ma;Xiao-Feng Zeng;Yong-Na Zhao;Dong-Xian Zhang;Yang Yu;Qian-Wen Yan;Yi-Qing Zhou;Shi-Jun Hong;Li-Hua Li
目的探讨天麻素（GAS）对甲基苯丙胺（MA）诱导的大鼠条件位置偏好（CPP）的影响及其可能机制。先给大鼠腹腔注射MA (10 mg/kg)，然后在第4天至第13天（D4-13天）交替给药MA或生理盐水，连续10天，然后在D15-21天给药GAS(10或20 mg/kg， i.p)，连续7天。大鼠经MA和GAS处理后进行CPP检测。体外将SH-SY5Y细胞暴露于MA (2.0 mM) 24 h后，再用GAS（2.0或4.0 mM）处理24 h。采用Western blot方法检测MA诱导CPP大鼠前额皮质、伏隔核和腹侧被盖区以及SH-SY5Y细胞中PKA、P-PKA、CREB和P-CREB蛋白的表达水平。成功建立ma诱导CPP大鼠模型。根据CPP方案测量，MA的使用刺激了行为的显著改变。经GAS处理后，大鼠在ma配对的室中度过的时间明显减少。结果还显示，MA增加了CPP大鼠前额皮质、伏隔核和腹侧被盖区以及SH-SY5Y细胞中PKA、p- PKA、CREB和p-CREB蛋白的表达水平（p< 0.05）。GAS能减弱ma诱导的CPP对大鼠的影响，降低体内和体外蛋白的表达水平。我们的研究表明，GAS可以通过调节PKA/CREB信号通路来减弱ma诱导的CPP对大鼠的影响。
To investigate the effects of gastrodin (GAS) on methamphetamine (MA)-induced conditioned place preference (CPP) in rats and explore its potential mechanisms. MA (10 mg/kg) was initially injected intraperitoneally (i.p.) in rats, after which they were administered either MA or saline alternately from day 4 to 13 (D4–13) for 10 days, followed by treatment with GAS (10 or 20 mg/kg, i.p.) on D15–21 for 7 days. The rats underwent CPP testing after MA and GAS treatment. In vitro, SH-SY5Y cells were exposed to MA (2.0 mM) for 24 h, followed by treatment with GAS (2.0 or 4.0 mM) for 24 h. The expression levels of PKA, P-PKA, CREB, and P-CREB proteins in the prefrontal cortex, nucleus accumbens, and ventral tegmental area of MA-induced CPP rats and in SH-SY5Y cells were detected by Western blot analysis. The MA-induced CPP rat model was successfully established. The administration of MA stimulated a significant alteration in behavior, as measured by the CPP protocol. After treatment with GAS, the amount of time rats spent in the MA-paired chamber was significantly reduced. Results also showed that MA increased the expression levels of PKA, P-PKA, CREB, and p-CREB proteins in the prefrontal cortex, nucleus accumbens, and ventral tegmental area of CPP rats and in SH-SY5Y cells (p< 0.05). GAS attenuated the effect of MA-induced CPP in rats and decreased the expression levels of proteins in vivo and in vitro. Our study suggests that GAS can attenuate the effects of MA-induced CPP in rats by regulating the PKA/CREB signaling pathway.
登录
查看更多内容
影响因子：
3.6
作者：
Mizoguchi, H;Yamada, K;Nabeshima, T
通讯作者：
Nabeshima, T
影响因子：
4.3
作者：
K. Zaitsu;I. Miyawaki;K. Bando;H. Horie;N. Shima;M. Katagi;M. Tatsuno;T. Bamba;Takako Sato;A. Ishii;H. Tsuchihashi;Koichi Suzuki;E. Fukusaki
通讯作者：
K. Zaitsu;I. Miyawaki;K. Bando;H. Horie;N. Shima;M. Katagi;M. Tatsuno;T. Bamba;Takako Sato;A. Ishii;H. Tsuchihashi;Koichi Suzuki;E. Fukusaki
影响因子：
4.2
作者：
M. Vandesquille;Mathieu Baudonnat;L. Decorte;C. Louis;P. Lestage;D. Béracochéa
通讯作者：
M. Vandesquille;Mathieu Baudonnat;L. Decorte;C. Louis;P. Lestage;D. Béracochéa
DOI：
10.1186/1755-7682-3-24
发表时间：
2010-10-06
期刊：
International archives of medicine
影响因子：
--
作者：
Arias-Carrión O;Stamelou M;Murillo-Rodríguez E;Menéndez-González M;Pöppel E
通讯作者：
Pöppel E
DOI：
--
发表时间：
2009-09
期刊：
International journal of physiology, pathophysiology and pharmacology
影响因子：
--
作者：
Benjamin C. Reiner;James P. Keblesh;H. Xiong
通讯作者：
Benjamin C. Reiner;James P. Keblesh;H. Xiong