Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.
Selection of growth-related genes and dominant genotypes in transgenic Yellow River carp Cyprinus carpio L.
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转基因黄河鲤鱼生长相关基因及显性基因型的筛选。
DOI：
10.1007/s10142-018-0597-9
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发表时间：
2018-07
影响因子：
2.9
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Luo L;Huang R;Zhang A;Yang C;Chen L;Zhu D;Li Y;He L;Liao L;Zhu Z;Wang Y
转基因的鲤鱼的特征是快速生长速度和高饲料转换效率，但是，在转基因黄河鲤鱼家族中，仍然存在与生长相关的基因型中的生长特征的显着分离；在具有显着的生长特征的转基因鲤鱼家族中，大量的分析（BSA）通过结合基因组结合研究（GWAS）的候选基因（GWAS），并鉴定出两个与生长有关的基因。翻译因子2-α激酶3（EIF2AK3），三个携带基因型17GG，17GG+14cc和17GG+14TT的亚组的平均体重增加了27.96、38.28和33.72％的体重，并分别增加了33.72％。和30.92％。结果表明，可以通过BSA测序从后代种群中选择适当的基因型载体，并结合简化的GWAS分析，因此可以构建基本的育种种群，并可以繁殖具有稳定的生产性能和均匀的生产性能。
本文的在线版本（10.1007/s10142-018-0597-9）包含补充材料，可供授权用户使用。
Transgenic Yellow River carp is characterized by rapid growth rate and high feed-conversion efficiency and exhibits a great application prospect. However, there is still a significant separation of growth traits in the transgenic Yellow River carp family; as such, growth-related genotypes must be screened for molecular marker-assisted selection. In this study, 23 growth-related candidate genes containing 48 SNP markers were screened through bulked segregant analysis (BSA) among transgenic Yellow River carp family members showing significant separation of growth traits. Then, two growth-related genes (Nos. 17 and 14 genes) were identified through combined genome-wide association study (GWAS) of candidate genes and validation of the full-sibling family approach. Nos. 17 and 14 genes encode BR serine/threonine-protein kinase 2 (BRSK2) and eukaryotic translation-initiation factor 2-alpha kinase 3 (Eif2ak3), respectively. The average body weight of three subgroups carrying the genotypes 17GG, 17GG + 14CC, and 17GG + 14TT of these two genes increased by 27.96, 38.28, and 33.72%, respectively, compared with the controls. The proportion of individuals with body weight > 500 g in these subgroups increased by 19.22, 26.82, and 30.92%, respectively. The results showed that appropriate genotype carriers can be selected from the progeny population through BSA sequencing combined with simplified GWAS analysis. Hence, basic population for breeding can be constructed and transgenic Yellow River carp strains with stable production performance and uniform phenotypic properties can be bred.
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