Methylmercury-induced changes in gene transcription associated with neuroendocrine disruption in largemouth bass (Micropterus salmoides).
Methylmercury-induced changes in gene transcription associated with neuroendocrine disruption in largemouth bass (Micropterus salmoides).
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DOI：
10.1016/j.ygcen.2014.03.029
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发表时间：
2014-07-01
影响因子：
2.7
通讯作者：
Tillitt, Donald E.
中科院分区：
文献类型：
--
作者：
Richter, Catherine A.;Martyniuk, Christopher J.;Annis, Mandy L.;Brumbaugh, William G.;Chasar, Lia C.;Denslow, Nancy D.;Tillitt, Donald E.
关键词：
甲基汞 (MeHg) 是一种强效神经内分泌干扰物，会损害鱼类的生殖过程。本研究的目的是 (1) 表征在受控实验室条件下雌性大口黑鲈 (LMB) 下丘脑暴露于甲基汞引起的转录组变化，(2) 研究自然环境中甲基汞暴露对雄性和雌性大口黑鲈 (LMB) 的健康和生殖影响，以及 (3) 确定来自甲基汞污染栖息地的雌性大口黑鲈 (LMB) 全脑中与甲基汞相关的基因表达模式。实验室实验是单次注射 2.5 μg MeHg/g 体重，暴露 96 小时。该实地调查比较了美国佛罗里达州鱼类甲基汞浓度相对较低的河流系统（韦基瓦河、圣达菲河和圣约翰河）和甲基汞浓度较高的 LMB 河流系统（圣玛丽河）。微阵列分析用于量化对甲基汞暴露的转录组反应。尽管高甲基汞地点的鱼类没有表现出明显的健康或生殖障碍，但实验室研究中发现了甲基汞响应基因和途径，相对于低甲基汞地点的鱼类，高甲基汞地点的鱼类也发生了改变。基因网络分析表明，MeHg 调节神经肽受体和类固醇信号传导的表达靶标，以及细胞的结构成分。根据表达数据，与甲基汞暴露相关的疾病相关基因网络包括小脑共济失调、运动障碍和高钙血症。中枢神经系统中的基因反应与已记录的甲基汞对脊椎动物的神经毒理学和神经内分泌干扰作用一致。
Methyl-mercury (MeHg) is a potent neuroendocrine disruptor that impairs reproductive processes in fish. The objectives of this study were to (1) characterize transcriptomic changes induced by MeHg exposure in the female largemouth bass (LMB) hypothalamus under controlled laboratory conditions, (2) investigate the health and reproductive impacts of MeHg exposure on male and female largemouth bass (LMB) in the natural environment, and (3) identify MeHg-associated gene expression patterns in whole brain of female LMB from MeHg-contaminated habitats. The laboratory experiment was a single injection of 2.5 μg MeHg/g body weight for 96 h exposure. The field survey compared river systems in Florida, USA with comparably lower concentrations of MeHg (Wekiva, Santa Fe, and St. Johns Rivers) in fish and one river system with LMB that contained elevated concentrations of MeHg (St. Marys River). Microarray analysis was used to quantify transcriptomic responses to MeHg exposure. Although fish at the high-MeHg site did not show overt health or reproductive impairment, there were MeHg-responsive genes and pathways identified in the laboratory study that were also altered in fish from the high-MeHg site relative to fish at the low-MeHg sites. Gene network analysis suggested that MeHg regulated the expression targets of neuropeptide receptor and steroid signaling, as well as structural components of the cell. Disease-associated gene networks related to MeHg exposure, based upon expression data, included cerebellum ataxia, movement disorders, and hypercalcemia. Gene responses in the CNS are consistent with the documented neurotoxicological and neuroendocrine disrupting effects of MeHg in vertebrates.
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影响因子：
2.7
作者：
Liu, Qing;Basu, Niladri;Goetz, Giles;Jiang, Nan;Hutz, Reinhold J.;Tonellato, Peter J.;Carvan, Michael J., III
通讯作者：
Carvan, Michael J., III
影响因子：
11.4
作者：
Chasar, Lia C.;Scudder, Barbara C.;Aiken, George R.
通讯作者：
Aiken, George R.
影响因子：
9.8
作者：
Adams, Douglas H.;Sonne, Christian;Jensen, Asger L.
通讯作者：
Jensen, Asger L.
影响因子：
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作者：
Cizdziel, J;Hinners, T;Pollard, J
通讯作者：
Pollard, J
影响因子：
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作者：
Hinck, Jo Ellen;Schmitt, Christopher J.;Tillitt, Donald E.
通讯作者：
Tillitt, Donald E.