Video bioinformatics analysis of human pluripotent stem cell morphology, quality, and cellular dynamics.
Video bioinformatics analysis of human pluripotent stem cell morphology, quality, and cellular dynamics.
复制标题
人类多能干细胞形态、质量和细胞动力学的视频生物信息学分析。
DOI：
10.1002/sctm.15-0352
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发表时间：
2021-09
影响因子：
6
通讯作者：
Talbot P
中科院分区：
文献类型：
--
作者：
Lin SC;Loza A;Antrim L;Talbot P
StemCellQC是一种视频生物信息学软件工具，用于定量分析人类多能干细胞（hPSC）集落。我们的目标是使用StemCellQC来评估和比较各种实验培养条件、细胞系和治疗方法，并证明其对PSC问题的适用性。确定了7个关键特征，提供了有用的信息PSC形态，动态行为和活力。层粘连蛋白-521上的集落附着优于Matrigel和Geltrex。当数据归一化时，每个基质上的生长率相似。亮度/面积比特征显示在Matrigel和Geltrex上生长的集落中的细胞死亡比在层粘连蛋白-521上生长的集落中的细胞死亡更大，这进一步促成了在层粘连蛋白-521上细胞的总体产量更高。四种不同的PSC培养基表现相似;然而，一种培养基在菌落形态和动态特征方面产生了批次间差异。两个胚胎和一个诱导多能干细胞系在形态、生长率、运动性和死亡率方面表现出显着差异。来自同一小瓶的在培养中变得表型不同的细胞在形态、亮度和运动性方面显示出可测量的差异。同样，分化和未分化集落的生长速度，强度和运动性不同。三种多能细胞系用低浓度的肉桂醛（一种用于消费品的化学物质）处理，显示出不良反应，并且对治疗的敏感性不同。我们的数据证明了StemCellQC的各种应用，可用于基础和转化研究，毒理学和药物测试以及从事干细胞治疗的临床设施。图形摘要显示StemCellQC的工作流程和功能。
StemCellQC is a video bioinformatics software tool for the quantitative analysis of human pluripotent stem cell (hPSC) colonies. Our objective was to use StemCellQC to evaluate and compare various experimental culture conditions, cell lines, and treatments and to demonstrate its applicability to PSC problems. Seven key features were identified that provided useful information on PSC morphology, dynamic behavior, and viability. Colony attachment was better on laminin‐521 than on Matrigel and Geltrex. Growth rates were similar on each matrix when data were normalized. The brightness/area ratio feature showed greater cell death in colonies grown on Matrigel and Geltrex than on laminin‐521 further contributing to an overall greater yield of cells on laminin‐521. Four different PSC culture media performed similarly; however, one medium produced batch‐to‐batch variation in colony morphology and dynamic features. Two embryonic and one induced pluripotent stem cell line showed significant differences in morphology, growth rates, motility, and death rates. Cells from the same vial that became phenotypically different in culture showed measurable differences in morphology, brightness, and motility. Likewise, differentiating and undifferentiated colonies varied in growth rate, intensity, and motility. Three pluripotent cell lines treated with a low concentration of cinnamaldehyde, a chemical used in consumer products, showed adverse effects and differed in their sensitivity to treatment. Our data demonstrate various applications of StemCellQC which could be used in basic and translational research, toxicological and drug testing, and clinical facilities engaged in stem cell therapy. Graphical abstract showing the workflow and features of StemCellQC.
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影响因子：
4.6
作者：
Phadnis SM;Loewke NO;Dimov IK;Pai S;Amwake CE;Solgaard O;Baer TM;Chen B;Reijo Pera RA
通讯作者：
Reijo Pera RA
影响因子：
4.6
作者：
Pang, Li
通讯作者：
Pang, Li
影响因子：
3.7
作者：
Zahedi A;On V;Lin SC;Bays BC;Omaiye E;Bhanu B;Talbot P
通讯作者：
Talbot P
DOI：
10.1007/978-3-319-23724-4_1
发表时间：
2015-01-01
期刊：
VIDEO BIOINFORMATICS: FROM LIVE IMAGING TO KNOWLEDGE
影响因子：
--
作者：
Bhanu, Bir;Talbot, Prue
通讯作者：
Talbot, Prue
影响因子：
5.2
作者：
Behar, Rachel Z.;Luo, Wentai;Talbot, Prue
通讯作者：
Talbot, Prue