Genotype diversity of H9N2 viruses isolated from wild birds and chickens in Hunan Province, China.
Genotype diversity of H9N2 viruses isolated from wild birds and chickens in Hunan Province, China.
复制标题
中国湖南省野生鸟类和鸡中分离的 H9N2 病毒基因型多样性
DOI：
10.1371/journal.pone.0101287
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Wang B;Liu Z;Chen Q;Gao Z;Fang F;Chang H;Chen J;Xu B;Chen Z
从洞庭湖湿地分离到3株H9 N2亚型禽流感病毒，其中1株来自新鲜白鹭粪便，2株来自家禽市场鸡泄泻腔拭子。系统发育分析表明，来自白鹭的H9 N2病毒的8个基因可能来自类似韩国或美国的谱系。这两株禽源H9 N2病毒是介于CK/BJ/94样病毒和G1样病毒之间的分离株。除PB 1基因（90.6%）外，其它内部基因的核苷酸序列同源性均在95%以上。此外，它们还与2013年在中国引起流行的H7N9病毒的一些基因表现出较高的同源性（96-98.3%）。禽源和白鹭源H9 N2病毒的核苷酸序列同源性较低。感染研究表明，白鹭源H9 N2病毒对小鼠和鸡均无致病性，病毒在肺内连续传8代也不能感染鸡。另一方面，感染禽源性病毒的鸡表现出明显的临床症状，甚至死亡;感染的小鼠没有明显的临床症状和体重下降，但在其肺部可以检测到病毒。总之，对于白鹭源H9 N2病毒，要实现家禽和哺乳动物的跨物种传播，需要一个漫长的适应过程。同一地理区域内同一宿主种不同时间分离的H9 N2病毒具有不同的进化状态，同一地理区域内不同宿主分离的病毒具有遗传多样性。因此，继续开展H9 N2病毒监测，了解其演变趋势，为疾病预防控制提供指导意义。
Three H9N2 avian influenza viruses were isolated from the Dongting Lake wetland, among which one was from fresh egret feces, the other two were from chicken cloacal swabs in poultry markets. Phylogenetic analyses suggested that eight genes of the egret-derived H9N2 virus might come from Korean-like or American-like lineages. The two poultry-derived H9N2 viruses were reassortants between the CK/BJ/94-like and G1-like viruses. Except the PB1 genes (90.6%), the nucleotide sequence of other internal genes of the two viruses exhibited high homology (>95%). In addition, they also exhibited high homology (96–98.3%) with some genes of the H7N9 virus that caused an epidemic in China in 2013. Nucleotide sequence of the poultry-derived and egret-derived H9N2 viruses shared low homology. Infection studies showed that the egret-derived H9N2 virus was non-pathogenic to both mice and chickens, and the virus was unable to infect chickens even through 8 passages continuously in the lung. On the other hand, the chickens infected by poultry-derived viruses showed obvious clinical symptoms and even died; the infected mice showed no noticeable clinical symptoms and weight loss, but viruses could be detected in their lungs. In conclusion, for the egret-derived H9N2 virus, it would take a long adaptation process to achieve cross-species transmission in poultry and mammals. H9N2 viruses isolated at different times from the same host species in the same geographical region presented different evolutionary status, and virus isolated from different hosts in the same geographical region exhibited genetic diversity. Therefore, it is important to continue the H9N2 virus surveillance for understanding their evolutionary trends so as to provide guidance for disease control and prevention.
登录
查看更多内容
人类感染新型禽源甲型流感 (H7N9) 病毒。
影响因子：
158.5
作者：
Gao, Rongbao;Cao, Bin;Shu, Yuelong
通讯作者：
Shu, Yuelong
影响因子：
2.7
作者：
Tosh, C.;Nagarajan, S.;Dubey, S. C.
通讯作者：
Dubey, S. C.
DOI：
10.1073/pnas.160270697
发表时间：
2000-08-15
影响因子：
11.1
作者：
Lin, YP;Shaw, M;Hay, A
通讯作者：
Hay, A
1994-2008年中国H9N2流感病毒基因型进化和抗原漂移
影响因子：
3.3
作者：
Sun, Yipeng;Pu, Juan;Liu, Jinhua
通讯作者：
Liu, Jinhua
影响因子：
3.8
作者：
Lang, Andrew S.;Kelly, Anke;Runstadler, Jonathan A.
通讯作者：
Runstadler, Jonathan A.