Morphological and pathological changes of Eustachian tube mucosa in an animal model of eosinophilic otitis media.
Morphological and pathological changes of Eustachian tube mucosa in an animal model of eosinophilic otitis media.
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嗜酸粒细胞性中耳炎动物模型咽鼓管黏膜的形态及病理变化
DOI：
10.1016/j.bjorl.2020.09.011
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发表时间：
2022-09
影响因子：
2.2
通讯作者：
Zhao, Shouqin
中科院分区：
文献类型：
--
作者：
Yang, Jinsong;Zhao, Chunli;Chen, Peiwei;Zhao, Shouqin
嗜酸性中耳炎是一种难治性中耳炎，也是一种相当常见的中耳疾病。然而，嗜酸性中耳炎的发病机制尚不清楚。目的观察嗜酸性中耳炎大鼠咽鼓管黏膜上皮的病理及超微结构变化，进一步探讨嗜酸性中耳炎的发病机制。动物在第0天腹膜内注射2000 mg卵清蛋白和100 mg氢氧化铝（明矾），然后在第7天和第14天注射100 mg卵清蛋白和100 mg明矾。然后，从第21天开始，通过每日鼻滴注100 mg卵清蛋白溶液并在右耳鼓室内注射0.1 mL卵清蛋白（1000 mg/mL）（A组，n = 80）和在左耳鼓室内注射0.1 mL生理盐水（对照组，B组，n = 80），持续14天，对它们进行局部加强。分别于麻醉后第35、38、41、43天解剖颞骨。扫描电镜、苏木精-伊红和甲苯胺蓝染色观察咽鼓管黏膜的病理形态学变化。并对炎性细胞和纤毛进行计数。A组咽鼓管上皮肿胀、增厚。纤毛排列紊乱，部分脱落。嗜酸性粒细胞浸润咽鼓管黏膜下层，数量明显增多，与B组比较有显著性差异（p < 0.05）。同时A组可见肥大细胞脱颗粒。扫描电镜观察发现A组纤毛沿着咽鼓管全长分布。纤毛细胞密度明显低于B组（p < 0.01）。在嗜酸性中耳炎模型中，变态反应引起咽鼓管黏膜的病理形态学改变，影响咽鼓管的正常功能，在嗜酸性中耳炎的发生发展中起重要作用。
Eosinophilic otitis media is an intractable otitis media and a fairly common middle ear disease. However, the pathogenesis of eosinophilic otitis media is obscure. To observe the pathological and ultrastructural changes of the Eustachian tube mucosal epithelium in rats with eosinophilic otitis media and further explore the pathogenesis of eosinophilic otitis media. Animals were intraperitoneally injected with 2000 mg ovalbumin and 100 mg aluminum hydroxide (alum) on day 0, followed by 100 mg ovalbumin and 100 mg alum injection on days 7 and 14. Next they were topically boosted by daily application of 100 mg ovalbumin solution via nasal drip and intratympanic injection of 0.1 mL ovalbumin (1000 mg/mL) in the right ear (group A, n = 80) and 0.1 mL saline in the left ear as control (group B, n = 80) starting on day 21 and continuing for 14 days. The temporal bones were dissected on the 35th, 38th, 41st and 43rd day separately under anesthesia. Scanning electron microscopy, hematoxylin-eosin and toluidine blue staining were used to observe the pathological and morphological changes of Eustachian tube mucosa stained samples. Moreover, inflammatory cells and cilia were counted. The epithelium of the Eustachian tube in group A was swollen and thickened. The cilia were arranged in a disorderly manner and partially detached. Eosinophils infiltrated the submucosal layer of the Eustachian tube, and their number increased significantly compared with that in group B (p < 0.05). Simultaneously, mast cell degranulation was observed in group A. Scanning electron microscopy revealed that the cilia were lodged and gathered along the whole length of Eustachian tube in group A. Ciliated cell density was significantly lower than that in Group B (p < 0.01). In the eosinophilic otitis media model, allergy caused significant changes in pathology and morphology of the Eustachian tube mucosa, affecting the normal function of the Eustachian tube which played an important role in the occurrence and development of eosinophilic otitis media.
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影响因子：
1.7
作者：
Iino, Yukiko;Tomioka-Matsutani, Sachiko;Nonaka, Manabu
通讯作者：
Nonaka, Manabu
DOI：
10.1016/j.ijporl.2012.09.039
发表时间：
2013-02-01
影响因子：
1.5
作者：
Kwon, Chul;Lee, Ho Yun;Yeo, Seung Geun
通讯作者：
Yeo, Seung Geun
影响因子：
1.7
作者：
Iino, Yukiko;Takahashi, Eli;Kikuchi, Saori
通讯作者：
Kikuchi, Saori
影响因子：
2.2
作者：
Martines, Francesco;Salvago, Pietro;Ferrara, Sergio;Messina, Giuseppe;Mucia, Marianna;Plescia, Fulvio;Sireci, Federico
通讯作者：
Sireci, Federico
影响因子：
3.7
作者：
Val S;Poley M;Brown K;Choi R;Jeong S;Colberg-Poley A;Rose MC;Panchapakesan KC;Devaney JC;Perez-Losada M;Preciado D
通讯作者：
Preciado D