Theranostic mesoporous silica nanoparticles biodegrade after pro-survival drug delivery and ultrasound/magnetic resonance imaging of stem cells.
Theranostic mesoporous silica nanoparticles biodegrade after pro-survival drug delivery and ultrasound/magnetic resonance imaging of stem cells.
复制标题
DOI：
10.7150/thno.11389
复制
发表时间：
2015
期刊：
影响因子：
12.4
通讯作者：
Jokerst JV
中科院分区：
文献类型：
--
作者：
Kempen PJ;Greasley S;Parker KA;Campbell JL;Chang HY;Jones JR;Sinclair R;Gambhir SS;Jokerst JV
在干细胞治疗中增加细胞存活是再生医学领域的一个重要挑战。在这里，我们报告了治疗诊断介孔二氧化硅纳米粒子，可以通过诊断和治疗方法增加细胞存活。首先，纳米颗粒提供超声和MRI信号，以引导植入到梗死周围区域并远离坏死组织。第二，纳米颗粒作为胰岛素样生长因子（IGF）的缓释库-一种显示增加细胞存活的蛋白质。用这些纳米颗粒标记的间充质干细胞的检测限接近9000个细胞，在标记所需的250 μg/mL浓度下没有细胞毒性。我们还研究了纳米颗粒的降解，并表明它们在大约3周内从细胞中清除。在体外无血清培养条件下，与未标记的细胞相比，IGF的存在使细胞存活率增加高达40%（p<0.05）。
Increasing cell survival in stem cell therapy is an important challenge for the field of regenerative medicine. Here, we report theranostic mesoporous silica nanoparticles that can increase cell survival through both diagnostic and therapeutic approaches. First, the nanoparticle offers ultrasound and MRI signal to guide implantation into the peri-infarct zone and away from the most necrotic tissue. Second, the nanoparticle serves as a slow release reservoir of insulin-like growth factor (IGF)—a protein shown to increase cell survival. Mesenchymal stem cells labeled with these nanoparticles had detection limits near 9000 cells with no cytotoxicity at the 250 µg/mL concentration required for labeling. We also studied the degradation of the nanoparticles and showed that they clear from cells in approximately 3 weeks. The presence of IGF increased cell survival up to 40% (p<0.05) versus unlabeled cells under in vitro serum-free culture conditions.
登录
查看更多内容
影响因子：
10.8
作者：
Burns AA;Vider J;Ow H;Herz E;Penate-Medina O;Baumgart M;Larson SM;Wiesner U;Bradbury M
通讯作者：
Bradbury M
影响因子：
6.7
作者：
Casciaro, Sergio;Conversano, Francesco;Gigli, Giuseppe
通讯作者：
Gigli, Giuseppe
影响因子：
7.8
作者：
George, Jon C.
通讯作者：
George, Jon C.
影响因子：
3.8
作者：
Kokhuis, T. J. A.;Skachkov, I.;de Jong, N.
通讯作者：
de Jong, N.
影响因子：
20.1
作者：
Haider, Husnain Kh;Jiang, Shujia;Ashraf, Muhammad
通讯作者：
Ashraf, Muhammad