Application of proteomics to Pseudomonas aeruginosa.
Application of proteomics to Pseudomonas aeruginosa.
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蛋白质组学在铜绿假单胞菌中的应用。
DOI：
10.1007/3-540-36459-5_5
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
S. Cordwell
中科院分区：
文献类型：
--
作者：
A. Nouwens;B. Walsh;S. Cordwell
最近假单胞菌基因组计划的完成，以及假单胞菌群落注释计划(PseudoCAP)的完成，使我们能够快速地将‘蛋白质组学’一词下的工具应用于这种病原体。这种全球方法将使研究界能够回答有关铜绿假单胞菌在不同生境中生存的能力、其对抗生素的高度内在抗药性以及它对人类的致病性质等长期存在的问题。蛋白质组学提供了一系列工具，能够确认开放阅读框架(ORF)的表达、其表达的相对水平、这种表达所需的环境条件以及编码基因产物的亚细胞定位。由于蛋白质是重要的细胞效应器，我们提出的生物学问题可以定义为蛋白质表达的变化，通过双向凝胶电泳法(2-DGE)分离到纯度可检测到的蛋白质表达的变化，以及通过质谱仪与基因序列的关系。因此，我们可以比较具有良好表型差异、在各种压力下的生长、蛋白质相互作用和复合体的菌株，并帮助定义未知功能的蛋白质。虽然全基因组最近才完成，但一些研究已经利用了这些信息，并使用蛋白质组学检查了各种蛋白质基因产物。本文综述了蛋白质组学在铜绿假单胞菌中的应用，并强调了未来研究的潜在领域，包括克服与2-DGE相关的传统技术限制。在2-DGE之前以亚细胞部分(‘亚蛋白质组’)为靶点的更有针对性的方法可以提供更多的功能信息。对目前和以前的铜绿假单胞菌蛋白质组学研究进行了综述，并从理论上考虑了亚蛋白质组学方法对加强这些研究的重要性。
The recent completion of the Pseudomonas Genome Project, in conjunction with the Pseudomonas Community Annotation Project (PseudoCAP) has fast-tracked our ability to apply the tools encompassed under the term 'proteomics' to this pathogen. Such global approaches will allow the research community to answer long-standing questions regarding the ability of Pseudomonas aeruginosa to survive diverse habitats, its high intrinsic resistance to antibiotics and its pathogenic nature towards humans. Proteomics provides an array of tools capable of confirming the expression of Open Reading Frames (ORF), the relative levels of their expression, the environmental conditions required for this expression and the sub-cellular location of the encoded gene-products. Since proteins are important cellular effectors, the biological questions we pose can be defined in terms of changes in protein expression detectable by separation to purity using two-dimensional gel electrophoresis (2-DGE) and relation to gene sequences via mass spectrometry. As such, we can compare strains with well-characterized phenotypic differences, growth under a variety of stresses, protein interactions and complexes and aid in defining proteins of unknown function. While the complete genome has only recently been finished, a number of studies have already utilized this information and examined various protein gene-products using proteomics. This review summarizes the application of proteomics to P. aeruginosa and highlights potential areas of future research, including overcoming the traditional technical limitations associated with 2-DGE. More focused approaches that target sub-cellular fractions ('sub-proteomes') prior to 2-DGE can provide further functional information. A review of current and previous proteomic projects on P. aeruginosa is presented, as well as theoretical considerations of the importance of sub-proteomic approaches to enhance these investigations.
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DOI：
10.1073/pnas.96.24.13904
发表时间：
1999-11-23
影响因子：
11.1
作者：
Whiteley, M;Lee, KM;Greenberg, EP
通讯作者：
Greenberg, EP
影响因子：
15.9
作者：
T. B. May;A. Chakrabarty
通讯作者：
T. B. May;A. Chakrabarty
DOI：
10.1099/00221287-146-10-2495
发表时间：
2000-10-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Hanna, SL;Sherman, NE;Goldberg, JB
通讯作者：
Goldberg, JB
影响因子：
15.9
作者：
Pesci, EC;Iglewski, BH
通讯作者：
Iglewski, BH