Chitosan/Alginate Nanoparticles with Sustained Release of Esculentoside A for Burn Wound Healing
Chitosan/Alginate Nanoparticles with Sustained Release of Esculentoside A for Burn Wound Healing
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壳聚糖/海藻酸盐纳米颗粒持续释放 Esculentoside A 用于烧伤创面愈合
DOI：
10.1021/acsanm.2c04714
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发表时间：
2023-01
影响因子：
5.9
通讯作者：
Chunmao Han
中科院分区：
文献类型：
--
作者：
Zhikang Zhu;Fang He;Huawei Shao;Jiaming Shao;Qiong Li;Xingang Wang;Haitao Ren;Chuangang You;Zhongtao Zhang;Chunmao Han
烧伤仍然是全球公共卫生最具破坏性的负担之一。烧伤和真皮支架移植引起的炎症往往导致烧伤创面延迟愈合。七叶皂苷A(ESA)具有很强的抗炎能力，是一种可能有助于烧伤创面治疗的有效药物。但ESA稳定性差、毒性大，限制了其临床应用。在本研究中，我们构建了壳聚糖/海藻酸盐纳米粒(ESA-CS/ALG-NPs)以提高可持续性和降低毒性，然后将制备的ESA-CS/ALG-NPs浸渍到胶原/壳聚糖支架(ESA-CS/ALG-NPs@CCS)中。用纳米计量仪、差示扫描量热仪、透射电子显微镜和傅立叶变换红外光谱分别对修复后的纳米粒子的粒径、结构形态、热性能和化学相互作用进行了表征。通过与RAW 264.7细胞的体外药物释放、生物相容性和抗炎能力，以及SD大鼠体内烧伤创面愈合实验，评价了复合ESA-CS/ALG-NPs@CCS的物理特性、体外药物释放、生物相容性和抗炎能力。结果表明，我们成功构建了CS/ALG-NPs，并对制备工艺进行了优化，以获得最高的包封率。ESA-CS/ALG-NPs@CCS具有较低的细胞毒性和ESA的缓释作用，在体外可减轻炎症反应，降低M1巨噬细胞比例，增加M2巨噬细胞比例。结果表明，5μg EsA CS/ALG-NPs@CCS不仅能减少炎性细胞因子的分泌，抑制M1巨噬细胞，还能促进抗炎细胞因子的释放，激活M2巨噬细胞，从而最终实现烧伤创面的快速优质愈合。综上所述，我们的工作表明，ESA、纳米颗粒和支架的协同组合为烧伤的治疗提供了一种有前途的策略。
Burn injury remains one of the most devastating burdens on global public health. The inflammation, caused by burn injury and transplantation of dermal scaffolds, often leads to delayed burn wound healing. Esculentoside A (EsA), with a strong anti-inflammatory capacity, is an available agent that might contribute to the treatment of burn wounds. However, the poor stability and toxicity of EsA limit its clinical application. In the present study, we constructed chitosan/alginate nanoparticles (EsA-CS/ALG-NPs) to improve sustainability and reduce toxicity followed by impregnation of the prepared EsA-CS/ALG-NPs into a collagen/chitosan scaffold (EsA-CS/ALG-NPs@CCS). The particle size, structural morphology, thermal properties, and chemical interaction of repaired nanoparticles were evaluated using the Nanometrics instrument, differential scanning calorimetry, transmission electron microscopy, and Fourier transform infrared spectroscopy, respectively. The hybrid EsA-CS/ALG-NPs@CCS was evaluated for physical characteristics, in vitro drug release, biocompatibility, and anti-inflammation capacity with RAW 264.7 cells and in vivo burn wound healing studies with SD rats. The results showed that we successfully constructed CS/ALG-NPs and optimized the preparation process to achieve the highest encapsulation efficiency. The hybrid EsA-CS/ALG-NPs@CCS, with reduced cytotoxicity and sustained release of EsA, could alleviate inflammation, decrease the ratio of M1 macrophages, and increase the proportion of M2 macrophages in vitro. It was demonstrated that 5 μg EsA CS/ALG-NPs@CCS not only reduces inflammatory cytokines secretion and inhibits M1 macrophages but also promotes the release of anti-inflammatory cytokines and activates M2 macrophages, thereby achieving accelerated and high-quality healing of burn wounds ultimately. In summary, our work suggests that the synergistic combination of EsA, nanoparticles, and scaffolds provided a promising strategy for treating burn injuries.
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影响因子：
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