Transcriptional regulation of thylakoid galactolipid biosynthesis coordinated with chlorophyll biosynthesis during the development of chloroplasts in Arabidopsis.
Transcriptional regulation of thylakoid galactolipid biosynthesis coordinated with chlorophyll biosynthesis during the development of chloroplasts in Arabidopsis.
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DOI：
10.3389/fpls.2014.00272
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发表时间：
2014
影响因子：
5.6
通讯作者：
Wada H
中科院分区：
文献类型：
--
作者：
Kobayashi K;Fujii S;Sasaki D;Baba S;Ohta H;Masuda T;Wada H
叶绿体中类囊体膜的生物发生需要叶绿素和光合蛋白与半乳糖脂单半乳糖二酰甘油（MGDG）和二半乳糖二酰甘油（DGDG）的协调合成，它们构成了类囊体脂质基质的主体。 MGD1和DGD1分别是MGDG和DGDG合成的关键酶。我们研究了拟南芥中 MGD1 和 DGD1 的表达谱，以确定在叶绿体生物发生过程中协调半乳糖脂合成与叶绿素和光合蛋白合成的转录调控。 MGD1 和 DGD1 的表达因叶绿素合成缺陷而受到抑制。此外，这些基因通过 GENOMES-UNCOUPLED1 介导的质体信号通路被氟磺草酮诱导的叶绿体功能下调。与其他光合作用相关核基因类似，MGD1和DGD1的表达是由光诱导的，其中细胞分裂素信号传导和LONG HYPOCOTYL5介导的光信号传导发挥着至关重要的作用。这些半乳糖脂合成基因的表达，特别是连续光下 DGD1 的表达，受到 GOLDEN2-LIKE 转录因子活性的强烈影响，GOLDEN2-LIKE 转录因子是叶绿素合成和叶绿体生物发生的有效调节因子。这些结果表明，在叶子发育过程中，半乳糖脂合成与光合叶绿素-蛋白质复合物的形成存在紧密的转录协调。同时，与光合基因不同，即使半乳糖脂与叶绿素一起积累，半乳糖脂合成基因在根部叶绿体生物发生过程中也没有上调，这表明根绿化过程中半乳糖脂合成的转录后调控的重要性。我们的数据表明，植物利用复杂的调节机制来改变植物生长过程中叶绿体发育的半乳糖脂合成。
Biogenesis of thylakoid membranes in chloroplasts requires the coordinated synthesis of chlorophyll and photosynthetic proteins with the galactolipids monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), which constitute the bulk of the thylakoid lipid matrix. MGD1 and DGD1 are the key enzymes of MGDG and DGDG synthesis, respectively. We investigated the expression profiles of MGD1 and DGD1 in Arabidopsis to identify the transcriptional regulation that coordinates galactolipid synthesis with the synthesis of chlorophyll and photosynthetic proteins during chloroplast biogenesis. The expression of both MGD1 and DGD1 was repressed in response to defects in chlorophyll synthesis. Moreover, these genes were downregulated by norflurazon-induced chloroplast malfunction via the GENOMES-UNCOUPLED1-mediated plastid signaling pathway. Similar to other photosynthesis-associated nuclear genes, the expression of MGD1 and DGD1 was induced by light, in which both cytokinin signaling and LONG HYPOCOTYL5-mediated light signaling played crucial roles. The expression of these galactolipid-synthesis genes, and particularly that of DGD1 under continuous light, was strongly affected by the activities of the GOLDEN2-LIKE transcription factors, which are potent regulators of chlorophyll synthesis and chloroplast biogenesis. These results suggest tight transcriptional coordination of galactolipid synthesis with the formation of the photosynthetic chlorophyll–protein complexes during leaf development. Meanwhile, unlike the photosynthetic genes, the galactolipid synthesis genes were not upregulated during chloroplast biogenesis in the roots, even though the galactolipids accumulated with chlorophylls, indicating the importance of post-transcriptional regulation of galactolipid synthesis during root greening. Our data suggest that plants utilize complex regulatory mechanisms to modify galactolipid synthesis with chloroplast development during plant growth.
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影响因子：
7.2
作者：
Fitter, DW;Martin, DJ;Langdale, JA
通讯作者：
Langdale, JA
影响因子：
4.8
作者：
Kelly, AA;Dörmann, P
通讯作者：
Dörmann, P
DOI：
10.1073/pnas.0704680104
发表时间：
2007-10-23
影响因子：
11.1
作者：
Kobayashi, Koichi;Kondo, Maki;Ohta, Hiroyuki
通讯作者：
Ohta, Hiroyuki
影响因子：
4.8
作者：
Dubots, Emmanuelle;Audry, Magali;Block, Maryse A.
通讯作者：
Block, Maryse A.
影响因子：
3.7
作者：
Hudson D;Guevara D;Yaish MW;Hannam C;Long N;Clarke JD;Bi YM;Rothstein SJ
通讯作者：
Rothstein SJ