Suppressed tumorigenicity of human endometrial cancer cells by the restored expression of the DCC gene.
Suppressed tumorigenicity of human endometrial cancer cells by the restored expression of the DCC gene.
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DOI：
10.1054/bjoc.1999.0943
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发表时间：
2000-01
影响因子：
8.8
通讯作者：
Barrett JC
中科院分区：
文献类型：
--
作者：
Kato H;Zhou Y;Asanoma K;Kondo H;Yoshikawa Y;Watanabe K;Matsuda T;Wake N;Barrett JC
为了获得DCC作为肿瘤抑制因子与子宫内膜癌相关的功能证据，我们将编码完整开放阅读框（ORF）的人DCC cDNA转染到高度致瘤性的人子宫内膜癌细胞、HHUA和Ishikawa中，在这些细胞中DCC的表达被完全删除。DCC在HHUA中的重组表达对体外生长影响不大，但能完全抑制小鼠肿瘤的形成。石川克隆的DCC表达丰富，与正常子宫内膜相似。体外生长受到抑制，呈现凋亡表型。在延长传代的克隆中，低水平的DCC表达不会诱导细胞凋亡，但仍有抑制致瘤性的潜力。这些观察结果暗示DCC在调节正常子宫内膜细胞生长中的作用，并将DCC归类为子宫内膜癌的肿瘤抑制基因。©2000癌症研究运动
To obtain functional evidence for DCC as a tumour suppressor associated with endometrial cancer, the human DCC cDNA encoding a complete open reading frame (ORF) was transfected into highly tumorigenic human endometrial carcinoma cells, HHUA and Ishikawa in which DCC expression was completely deleted. Reconstituted expression of DCC in HHUA had little effect on in vitro growth, but suppressed tumour formation in mice completely. The clones from Ishikawa had abundant DCC expression similar to that in normal endometrium. Their growth in vitro was suppressed and showed apoptotic phenotype. Lower levels of DCC expression in the prolonged passaged clones did not induce apoptosis, but still had the potential to suppress tumorigenicity. These observations imply a role of DCC in regulation of normal endometrial cell growth, and categorize DCC as the tumour suppressor gene for endometrial cancer. © 2000 Cancer Research Campaign
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影响因子：
8.8
作者：
Brewster, S. F.;Gingell, J. C.;Browne, S.;Brown, K. W.
通讯作者：
Brown, K. W.
影响因子：
64.5
作者：
KeinoMasu, K;Masu, M;TessierLavigne, M
通讯作者：
TessierLavigne, M
影响因子：
4
作者：
OSHIMURA, M;KUGOH, H;BARRETT, JC
通讯作者：
BARRETT, JC
DOI：
10.1073/pnas.95.8.4646
发表时间：
1998-04-14
影响因子：
11.1
作者：
Clifton, DR;Goss, RA;Sporn, LA
通讯作者：
Sporn, LA
影响因子：
64.8
作者：
Fazeli, A;Dickinson, SL;Weinberg, RA
通讯作者：
Weinberg, RA