Differentially expressed immune response genes in COVID-19 patients based on disease severity.
Differentially expressed immune response genes in COVID-19 patients based on disease severity.
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根据疾病严重程度，COVID-19 患者中差异表达的免疫反应基因
DOI：
10.18632/aging.202877
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发表时间：
2021-03-29
期刊：
影响因子：
--
通讯作者：
Li X
中科院分区：
文献类型：
--
作者：
Li S;Duan X;Li Y;Li M;Gao Y;Li T;Li S;Tan L;Shao T;Jeyarajan AJ;Chen L;Han M;Lin W;Li X
背景资料：对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的免疫反应失调被认为是2019冠状病毒病（COVID-19）进展的基础。我们试图根据疾病严重程度进一步表征COVID-19患者中的宿主抗病毒和细胞因子基因表达。研究方法：在这项病例对照研究中，我们回顾性分析了从阜阳市第二人民医院招募的46名康复的COVID-19患者和24名健康受试者（无COVID-19病史）。从每名研究参与者中采集血液样品用于RNA提取和PCR。我们评估了健康对照组与轻度/中度（MM）和重度/危重（SC）疾病患者之间抗病毒基因表达的变化。结果如下：我们发现，I型干扰素信号（IFNA 2、TLR 8、IFNA 1、IFNAR 1、TLR 9、IRF 7、ISG 15、APOBEC 3G和MX 1）和编码促炎细胞因子（IL 12 B、IL 15、IL 6、IL 12 A和IL 1B）和趋化因子（CXCL 9、CXCL 11和CXCL 10）的基因在MM和SC疾病患者中上调。此外，我们发现，与MM组相比，SC组中IFNA 1、载脂蛋白B mRNA编辑酶、催化多肽样3G（APOBEC 3G）和Fas相关蛋白死亡结构域（FADD）显著下调（P < 0.05）。我们还观察到MM组的microRNA（miR）-155和miR-130 a水平显著高于SC组。结论：COVID-19与宿主抗病毒基因的激活有关。IFN系统的诱导似乎在控制SARS-CoV-2感染中特别重要，因为相对于MM患者，SC患者中IFNA 1、APOBEC 3G和FADD基因的表达降低可能与疾病进展相关。
Background: Dysregulated immune responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are thought to underlie the progression of coronavirus disease 2019 (COVID-19). We sought to further characterize host antiviral and cytokine gene expression in COVID-19 patients based on illness severity. Methods: In this case-control study, we retrospectively analyzed 46 recovered COVID-19 patients and 24 healthy subjects (no history of COVID-19) recruited from the Second People's Hospital of Fuyang City. Blood samples were collected from each study participant for RNA extraction and PCR. We assessed changes in antiviral gene expression between healthy controls and patients with mild/moderate (MM) and severe/critical (SC) disease. Results: We found that type I interferon signaling (IFNA2, TLR8, IFNA1, IFNAR1, TLR9, IRF7, ISG15, APOBEC3G, and MX1) and genes encoding proinflammatory cytokines (IL12B, IL15, IL6, IL12A and IL1B) and chemokines (CXCL9, CXCL11 and CXCL10) were upregulated in patients with MM and SC disease. Moreover, we found that IFNA1, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3G (APOBEC3G), and Fas-associated protein with death domain (FADD) were significantly downregulated (P < 0.05) in the SC group compared to the MM group. We also observed that microRNA (miR)-155 and miR-130a levels were markedly higher in the MM group compared to the SC group. Conclusion: COVID-19 is associated with the activation of host antiviral genes. Induction of the IFN system appears to be particularly important in controlling SARS-CoV-2 infection, as decreased expression of IFNA1, APOBEC3G and FADD genes in SC patients, relative to MM patients, may be associated with disease progression.
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影响因子：
158.5
作者：
Li, Qun;Guan, Xuhua;Feng, Zijian
通讯作者：
Feng, Zijian
影响因子：
5.6
作者：
Ebrahimi N;Aslani S;Babaie F;Hemmatzadeh M;Hosseinzadeh R;Joneidi Z;Mehdizadeh Tourzani Z;Pakravan N;Mohammadi H
通讯作者：
Mohammadi H
影响因子：
7.3
作者：
Alivernini S;Gremese E;McSharry C;Tolusso B;Ferraccioli G;McInnes IB;Kurowska-Stolarska M
通讯作者：
Kurowska-Stolarska M
DOI：
10.18632/aging.103931
发表时间：
2020-10-14
期刊：
Aging
影响因子：
--
作者：
Liao Y;Feng Y;Wang B;Wang H;Huang J;Wu Y;Wu Z;Chen X;Yang C;Fu X;Sun H
通讯作者：
Sun H
影响因子：
3.8
作者：
Ki, Moran
通讯作者：
Ki, Moran