Monitoring of chronic wasting disease (CWD) (IV).
Monitoring of chronic wasting disease (CWD) (IV).
复制标题
DOI：
10.2903/j.efsa.2023.7936
复制
发表时间：
2023-04
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
欧盟委员会要求对挪威、瑞典、芬兰、冰岛、爱沙尼亚、拉脱维亚、立陶宛和波兰的慢性消耗性疾病（CWD）监测计划（2017年1月9日至2022年2月28日）进行分析。驯鹿中检测到 13 例病例，驼鹿中检测到 15 例，马鹿中检测到 3 例。他们表现出两种表型，通过淋巴网状组织中是否存在可检测到的与疾病相关的正常细胞朊病毒蛋白（PrP）来区分。 CWD 首次在芬兰、瑞典和挪威其他地区被发现。在没有发现这种疾病的国家，证据不足以完全排除它的存在。在发现病例的地方，患病率低于 1%。数据还表明，应修改监测的高风险目标群体，并删除“道路杀戮”。数据显示，除了年龄和性别差异外，阳性和阴性野生驯鹿之间的朊病毒蛋白基因（PRNP）基因型也存在差异。已提出逐步框架，扩大最低背景监测范围，在欧洲国家实施相关鹿科动物物种。额外的监测可能包括针对四个不同目标的特别调查，具体针对有/无病例的国家，重点是对高风险目标群体中成年鹿的肥胖和淋巴结进行平行检测，并使用抽样单位和数据驱动的设计患病率，持续一段时间。根据地理区域的定义、每年的引入风险评估、持续的最低背景监测、利益相关者的培训和参与以及基于数据驱动参数的监测计划，概述了评估 CWD 存在概率的标准。所有阳性病例均应进行基因分型。已经提出了负样本的样本大小来检测和估计 PRNP 多态性的频率。应对所有选定的样本进行整个 PRNP 开放阅读框的双链测序，并在欧盟层面的集中收集系统中整理数据。
The European Commission requested an analysis of the Chronic Wasting Disease (CWD) monitoring programme in Norway, Sweden, Finland, Iceland, Estonia, Latvia, Lithuania and Poland (9 January 2017–28 February 2022). Thirteen cases were detected in reindeer, 15 in moose and 3 in red deer. They showed two phenotypes, distinguished by the presence or absence of detectable disease‐associated normal cellular prion protein (PrP) in lymphoreticular tissues. CWD was detected for the first time in Finland, Sweden and in other areas of Norway. In countries where the disease was not detected, the evidence was insufficient to rule out its presence altogether. Where cases were detected, the prevalence was below 1%. The data also suggest that the high‐risk target groups for surveillance should be revised, and ‘road kill’ removed. Data show that, in addition to differences in age and sex, there are differences in the prion protein gene (PRNP) genotypes between positive and negative wild reindeer. A stepwise framework has been proposed with expanded minimum background surveillance to be implemented in European countries with relevant cervid species. Additional surveillance may include ad hoc surveys for four different objectives, specific to countries with/without cases, focusing on parallel testing of obex and lymph nodes from adult cervids in high‐risk target groups, sustained over time, using sampling units and a data‐driven design prevalence. Criteria for assessing the probability of CWD presence have been outlined, based on the definition of the geographical area, an annual assessment of risk of introduction, sustained minimum background surveillance, training and engagement of stakeholders and a surveillance programme based on data‐driven parameters. All positive cases should be genotyped. Sample sizes for negative samples have been proposed to detect and estimate the frequency of PRNP polymorphisms. Double‐strand sequencing of the entire PRNP open reading frame should be undertaken for all selected samples, with data collated in a centralised collection system at EU level.
登录
查看更多内容
DOI：
10.2903/j.efsa.2019.5925
发表时间：
2019-12-01
期刊：
EFSA journal. European Food Safety Authority
影响因子：
--
作者：
通讯作者：
--
影响因子：
6.7
作者：
Bian J;Kim S;Kane SJ;Crowell J;Sun JL;Christiansen J;Saijo E;Moreno JA;DiLisio J;Burnett E;Pritzkow S;Gorski D;Soto C;Kreeger TJ;Balachandran A;Mitchell G;Miller MW;Nonno R;Vikøren T;Våge J;Madslien K;Tran L;Vuong TT;Benestad SL;Telling GC
通讯作者：
Telling GC
影响因子：
4.1
作者：
Cullingham, Catherine I.;Merrill, Evelyn H.;Coltman, David W.
通讯作者：
Coltman, David W.
影响因子：
3.5
作者：
Brayton, KA;O'Rourke, KI;Knowles, DP
通讯作者：
Knowles, DP
影响因子：
2.6
作者：
Cameron, A. R.
通讯作者：
Cameron, A. R.