Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning.
Engineering Anisotropic Muscle Tissue using Acoustic Cell Patterning.
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DOI：
10.1002/adma.201802649
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发表时间：
2018-10
期刊：
影响因子：
--
通讯作者：
Stevens MM
中科院分区：
文献类型：
--
作者：
Armstrong JPK;Puetzer JL;Serio A;Guex AG;Kapnisi M;Breant A;Zong Y;Assal V;Skaalure SC;King O;Murty T;Meinert C;Franklin AC;Bassindale PG;Nichols MK;Terracciano CM;Hutmacher DW;Drinkwater BW;Klein TJ;Perriman AW;Stevens MM
组织工程为疾病建模和再生医学提供了独特的机会；然而，这些策略的成功依赖于原生细胞组织的忠实繁殖。在这里，据报道，超声驻波可以用来组织成肌细胞群体在材料系统中排列肌肉组织结构的工程。使用I型胶原水凝胶设计的图案肌肉在拉伸强度上表现出显著的各向异性，并且在机械约束下，在细胞和纤维水平上产生了微尺度的排列。此外，明胶甲基丙烯酰水凝胶中成肌细胞的声学模式显著增强了肌纤维的形成，并促进了含有肌管束的肌肉纤维的形成，肌管束的宽度为120-150µm，间距为180-220µm。在没有任何材料线索或复杂的制造过程的情况下远程排列肌管纤维的能力代表了肌肉组织工程领域的重大进步。总的来说，这些结果是由声学模式细胞分化形成的工程细胞纤维的第一个实例。预计这种多用途的方法可以应用于许多复杂的组织形态，与空间组织细胞培养，类器官发育和生物电子学具有更广泛的相关性。
Tissue engineering has offered unique opportunities for disease modeling and regenerative medicine; however, the success of these strategies is dependent on faithful reproduction of native cellular organization. Here, it is reported that ultrasound standing waves can be used to organize myoblast populations in material systems for the engineering of aligned muscle tissue constructs. Patterned muscle engineered using type I collagen hydrogels exhibits significant anisotropy in tensile strength, and under mechanical constraint, produced microscale alignment on a cell and fiber level. Moreover, acoustic patterning of myoblasts in gelatin methacryloyl hydrogels significantly enhances myofibrillogenesis and promotes the formation of muscle fibers containing aligned bundles of myotubes, with a width of 120–150 µm and a spacing of 180–220 µm. The ability to remotely pattern fibers of aligned myotubes without any material cues or complex fabrication procedures represents a significant advance in the field of muscle tissue engineering. In general, these results are the first instance of engineered cell fibers formed from the differentiation of acoustically patterned cells. It is anticipated that this versatile methodology can be applied to many complex tissue morphologies, with broader relevance for spatially organized cell cultures, organoid development, and bioelectronics.
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