Enzyme-based paper test for detection of lactose in illicit drugs
Enzyme-based paper test for detection of lactose in illicit drugs
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用于检测非法药物中乳糖的酶纸测试
DOI：
10.1039/c9ay02459j
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发表时间：
2020
影响因子：
3.1
通讯作者：
Lieberman, Marya
中科院分区：
文献类型：
--
作者：
Zinna, Jessica;Lockwood, Tracy-Lynn E.;Lieberman, Marya
乳糖通常被用作非法药物的切割剂。目前，用于非法药物的假定现场颜色检测试剂盒不检测乳糖或其他切割剂的存在。建立了一种在纸基测试卡上检测乳糖的方法。由乳糖酶、葡萄糖氧化酶和过氧化物酶组成的三酶系统用于将乳糖分解成过氧化物，然后用氧化还原指示剂检测。该测试可以检测固体样品中低至5%的乳糖浓度，并且当乳糖与非法药物或商业药物混合时不会产生干扰。制备的检测卡在货架上可稳定储存长达5个月。在由海洛因、甲基苯丙胺、可卡因盐酸盐、快克可卡因、填充剂和乳糖的混合物组成的样本的盲法研究中，三名读片员检测乳糖的灵敏度为100%，特异性为96.4%（n = 96）。当该测试被纳入一个12道检测卡检测非法药物，读者能够正确地识别非法药物和乳糖的存在与99.3%的灵敏度和100%的特异性（n = 54）。该测试是检测非法药物样品中乳糖的一种可靠且经济实惠的方法。
Lactose is commonly used as a cutting agent in illicit drugs. Currently, presumptive field color test kits for illicit drugs do not test for the presence of lactose or other cutting agents. A method was developed to detect lactose on a paper-based test card. A three-enzyme system comprised of lactase, glucose oxidase, and peroxidase was used to break down lactose into peroxide, which was then detected with a redox indicator. The test can detect lactose concentrations as low as 5% in solid samples and shows no interference when lactose is mixed with illicit drugs or commercial pharmaceuticals. Prepared test cards were stable on the shelf for up to five months. In a blinded study of samples composed of mixtures of heroin, methamphetamine, cocaine HCl, crack cocaine, fillers, and lactose, the sensitivity for detection of lactose across three readers was 100% and specificity was 96.4% (n = 96). When this test was incorporated into a 12-lane test card for the detection of illicit drugs, readers were correctly able to identify the illicit drug and the presence of lactose with 99.3% sensitivity and 100% specificity (n = 54). This test is a robust and affordable way to detect lactose in illicit drug samples.
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影响因子：
7.4
作者：
Weaver, Abigail A.;Reiser, Hannah;Barstis, Toni;Benvenuti, Michael;Ghosh, Debarati;Hunckler, Michael;Joy, Brittney;Koenig, Leah;Raddell, Kellie;Lieberman, Marya
通讯作者：
Lieberman, Marya
用于受控物质推定测试的微流体装置*
影响因子：
1.6
作者：
S. Bell;Rebecca D. Hanes
通讯作者：
Rebecca D. Hanes
DOI：
--
发表时间：
1975
期刊：
影响因子：
--
作者：
Perry Dc
通讯作者：
Perry Dc
影响因子：
2.7
作者：
Sandeep Sharma;N. Sehgal;Ashok Kumar
通讯作者：
Sandeep Sharma;N. Sehgal;Ashok Kumar
影响因子：
3.1
作者：
Jamie L. Luther;Valentine Henry de Frahan;M. Lieberman
通讯作者：
M. Lieberman