Native Desorption Electrospray Ionization Liberates Soluble and Membrane Protein Complexes from Surfaces
Native Desorption Electrospray Ionization Liberates Soluble and Membrane Protein Complexes from Surfaces
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自然解吸电喷雾电离从表面释放可溶性和膜蛋白复合物
DOI：
10.1002/ange.201704849
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发表时间：
2017
影响因子：
--
通讯作者：
Ambrose S
中科院分区：
文献类型：
--
作者：
Ambrose S
Mass spectrometry (MS) applications for intact protein complexes typically require electrospray (ES) ionization and have not been achieved via direct desorption from surfaces. Desorption ES ionization (DESI) MS has however transformed the study of tissue surfaces through release and characterisation of small molecules. Motivated by the desire to screen for ligand binding to intact protein complexes we report the development of a native DESI platform. By establishing conditions that preserve non‐covalent interactions we exploit the surface to capture a rapid turnover enzyme–substrate complex and to optimise detergents for membrane protein study. We demonstrate binding of lipids and drugs to membrane proteins deposited on surfaces and selectivity from a mix of related agonists for specific binding to a GPCR. Overall therefore we introduce this native DESI platform with the potential for high‐throughput ligand screening of some of the most challenging drug targets including GPCRs.
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影响因子：
7.4
作者：
Liu, Pengyuan;Zhang, Jiang;Ferguson, Carly N.;Chen, Hao;Loo, Joseph A.
通讯作者：
Loo, Joseph A.
影响因子：
3.9
作者：
Borysik, Antoni J.;Robinson, Carol V.
通讯作者：
Robinson, Carol V.
DOI：
10.1016/j.bbalip.2011.05.006
发表时间：
2011-11
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Eberlin LS;Ferreira CR;Dill AL;Ifa DR;Cooks RG
通讯作者：
Cooks RG
影响因子：
7.4
作者：
Ferguson, Carly N.;Benchaar, Sabrina A.;Miao, Zhixin;Loo, Joseph A.;Chen, Hao
通讯作者：
Chen, Hao
影响因子：
11.2
作者：
Abbassi-Ghadi, Nima;Golf, Ottmar;Hanna, George B.
通讯作者：
Hanna, George B.