DNA probe detection of periodontopathogens in advanced periodontitis.
DNA probe detection of periodontopathogens in advanced periodontitis.
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DNA探针检测晚期牙周炎中的牙周病原体。
DOI：
10.1111/j.1600-0722.1993.tb01133.x
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
B. Söder
中科院分区：
文献类型：
--
作者：
P. Söder;L. Jin;B. Söder
使用种特异性DNA探针来确定伴放线放线杆菌（Actinobacillus actinomycetemcomitans，A.a.）的存在，牙龈卟啉单胞菌（拟杆菌）、中间普雷沃菌、齿垢密螺旋体、腐蚀艾肯菌、具核梭杆菌和直窝菌在晚期牙周炎患者深袋/部位的龈下菌斑中的分布。受试者为20例重度成人牙周炎患者，13例男性和7例女性（平均年龄45.6 ± 6.7岁）。对于每个受试者，通过纸点法从每个象限中取样9 - 10个具有最深探测深度的龈下部位，总共198个部位，平均探测深度为7.2 +/-1.6 mm，临床附着水平为9.5 +/-2.7 mm。在75%的受试者中存在至少一个部位;在95%的受试者中发现牙龈卟啉单胞菌;直肌的检出率为90%，T. denticola、齿生E. corrosodens和F.所有受试者均发现有核质。在198个样品中，A.a.牙龈卟啉单胞菌51.5%，中间卟啉单胞菌64.1%，T.齿垢E. 72.9%的F.具核的占74.7%;直肠占65.7%。优势组合是同时存在中间疫霉、T. denticola、齿生E. corrodens，F. nucleatum，W. 89.5%的受试者和46.8%的部位有直肠。在这些位点中，51.1%显示牙龈卟啉单胞菌的组合存在，28.4%显示A.a.和牙龈卟啉单胞菌。在14.4%的采样点中，7种细菌均未检出。目前的研究表明，严重破坏性成人牙周炎是一种多细菌感染，牙周病原体的某些组合似乎是重要的疾病的发病机制。
Species-specific DNA probes were used to determine the presence of Actinobacillus actinomycetemcomitans (A.a.), Porphyromonas (Bacteroides) gingivalis, Prevotella intermedia, Treponema denticola, Eikenella corrodens, Fusobacterium nucleatum, and Wolinella recta in subgingival plaque from deep pockets/sites of patients with advanced periodontitis. The subjects were 20 patients with severe adult periodontitis, 13 men and 7 women (mean age 45.6 +/- 6.7 yr). For each subject, 9-10 subgingival sites with the deepest probing depths from each quadrant were sampled by the paper point method, a total of 198 sites, with mean probing depth 7.2 +/- 1.6 mm and clinical attachment level 9.5 +/- 2.7 mm. A.a. was present in at least one site in 75% of the subjects; P. gingivalis was found in 95%; P. intermedia and W. recta were found in 90%, respectively; and T. denticola, E. corrodens, and F. nucleatum were found in all subjects. In the 198 samples, A.a. was detected in 25.8%, P. gingivalis in 51.5%, P. intermedia in 64.1%, T. denticola in 60.6%, E. corrodens in 72.9%, F. nucleatum in 74.7%, and W. recta in 65.7%. The predominant combination was the simultaneous presence of P. intermedia, T. denticola, E. corrodens, F. nucleatum, and W. recta in 89.5% of the subjects and 46.8% of the sites. Of these sites, 51.1% showed the combined presence of P. gingivalis and 28.4% that of both A.a. and P. gingivalis. None of the seven bacteria could be detected in 14.4% of the total sites sampled. The present study indicates that severe destructive adult periodontitis is a multibacterial infection and that certain combinations of periodontopathogens seem to be important in the pathogenesis of the disease.
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影响因子：
--
作者：
Genco,RJ;Zambon,JJ;Christersson,LA
通讯作者：
Christersson,LA
手术加强力霉素对幼年牙周炎的微生物学和临床效果。
影响因子：
4.3
作者：
Mandell,RL;Socransky,SS
通讯作者：
Socransky,SS
DNA探针检测龈下菌斑中的腐蚀艾肯氏菌、直肠沃利氏菌和具核梭杆菌。
影响因子：
--
作者：
Lippke,JA;Peros,WJ;Keville,MW;Savitt,ED;French,CK
通讯作者：
French,CK
某些龈下微生物物种在选定牙周条件下的分布。
影响因子：
3.5
作者：
Savitt,ED;Socransky,SS
通讯作者：
Socransky,SS
在寻找破坏性牙周病的病因中遇到了困难。
影响因子：
6.7
作者：
Socransky,SS;Haffajee,AD;Smith,GL;Dzink,JL
通讯作者：
Dzink,JL