Anaerobic soil disinfestation, amendment-type, and irrigation regimen influence Salmonella survival and die-off in agricultural soils.
Anaerobic soil disinfestation, amendment-type, and irrigation regimen influence Salmonella survival and die-off in agricultural soils.
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DOI：
10.1111/jam.15324
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发表时间：
2022-03
影响因子：
4
通讯作者：
Strawn LK
中科院分区：
文献类型：
--
作者：
Murphy CM;Weller DL;Reiter MS;Bardsley CA;Eifert J;Ponder M;Rideout SL;Strawn LK
这项研究调查了包括厌氧土壤消毒(ASD)、土壤改良类型和灌溉制度在内的园艺措施组合后的沙门氏菌浓度。沙壤土接种5个血清型沙门氏菌鸡尾酒(5.5±0.2logCFU g-1)，并接受六个处理之一：(I)不改良土壤，ASD(ASD对照)，(Ii)不改良土壤，不ASD(非ASD对照)，(III-vi)用家禽颗粒、黑麦、油菜籽或毛豌豆和ASD改良土壤。通过在灌水后3d和7d采集样本来确定灌水方案的效果。在ASD前、土壤饱和后(即ASD过程)和ASD后14个时间点收集了25克土壤样本，并列举了沙门氏菌水平。对数线性模型检验了改良类型和灌溉方式对沙门氏菌在ASD期间和ASD后死亡的影响。在ASD期间，所有处理的沙门氏菌浓度都显著下降(范围：−0.2~−2.7logcfu g−1)，但降幅最小(在颗粒禽窝中观察到的−0.2logcfu g-1)可以忽略不计。沙门氏菌消失率因修正而异，ASD后的平均死亡率为−0.05logCFU g-1day-1(CI=−0.05，−0.04)。在ASD后42天，沙门氏菌浓度在颗粒家禽粪便中保持最高，其次是油菜籽和毛豌豆处理。研究结果表明，ASD不能消除土壤中的沙门氏菌，某些土壤改良剂有助于提高沙门氏菌的存活率。与其他血清型相比，支持新港沙门氏菌存活的沙门氏菌血清型的分布因使用颗粒家禽的处理而不同。灌水对沙门氏菌的存活有促进作用，灌水后3d的沙门氏菌浓度为0.14logcfu g-1(CI=0.05，0.23)，高于灌水后7d。ASD并不能消除土壤中的沙门氏菌，事实上，根据所使用的土壤改良剂，可能会促进沙门氏菌的存活。应考虑实施园艺措施对食品安全危害的协同和拮抗效应。
This study investigated Salmonella concentrations following combinations of horticultural practices including anaerobic soil disinfestation (ASD), soil amendment type, and irrigation regimen. Sandy-loam soil was inoculated with a five-serovar Salmonella cocktail (5.5±0.2 log CFU g-1) and subjected to one of six treatments: (i) no soil amendment, ASD (ASD control), (ii) no soil amendment, no-ASD (non-ASD control), and (iii-vi) soil amended with pelletized poultry litter, rye, rapeseed, or hairy vetch with ASD. The effect of irrigation regimen was determined by collecting samples 3 and 7 d after irrigation. Twenty-five-gram soil samples were collected pre-ASD, post-soil saturation (i.e., ASD-process), and at 14 time-points post-ASD, and Salmonella levels enumerated. Log-linear models examined the effect of amendment type and irrigation regimen on Salmonella die-off during and post-ASD. During ASD, Salmonella concentrations significantly decreased in all treatments (range: −0.2 to −2.7 log CFU g−1), albeit the smallest decrease (−0.2 log CFU g-1 observed in the pelletized poultry litter) was of negligible magnitude. Salmonella die-off rates varied by amendment with an average post-ASD rate of −0.05 log CFU g-1 day-1 (CI=−0.05, −0.04). Salmonella concentrations remained highest over the 42 d post-ASD in pelletized poultry litter, followed by rapeseed, and hairy vetch treatments. Findings suggested ASD was not able to eliminate Salmonella in soil, and certain soil amendments facilitated enhanced Salmonella survival. Salmonella serovar distribution differed by treatment with pelletized poultry litter supporting S. Newport survival, compared to other serovars. Irrigation appeared to assist Salmonella survival with concentrations being 0.14 log CFU g-1 (CI=0.05, 0.23) greater 3 d, compared to 7 d post-irrigation. ASD does not eliminate Salmonella in soil, and may in fact, depending on the soil amendment used, facilitate Salmonella survival. Synergistic and antagonistic effects on food safety hazards of implementing horticultural practices should be considered.
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影响因子：
5.2
作者：
Bell, Rebecca L.;Zheng, Jie;Brown, Eric W.
通讯作者：
Brown, Eric W.
影响因子：
2
作者：
Guo, X;Chen, JR;Beuchat, LR
通讯作者：
Beuchat, LR
影响因子：
5.2
作者：
Bardsley CA;Weller DL;Ingram DT;Chen Y;Oryang D;Rideout SL;Strawn LK
通讯作者：
Strawn LK
影响因子：
11.8
作者：
Galanis, E;Wong, DMALF;WHO Global Salm Surv
通讯作者：
WHO Global Salm Surv
影响因子：
11.8
作者：
Braden, Christopher R.
通讯作者：
Braden, Christopher R.