LncRNA SNHG17 Contributes to Proliferation, Migration, and Poor Prognosis of Hepatocellular Carcinoma.
LncRNA SNHG17 Contributes to Proliferation, Migration, and Poor Prognosis of Hepatocellular Carcinoma.
复制标题
LncRNA SNHG17 导致肝细胞癌的增殖、迁移和不良预后
DOI：
10.1155/2021/9990338
复制
发表时间：
2021
影响因子：
2.7
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Luo Y;Lin J;Zhang J;Song Z;Zheng D;Chen F;Zhuang X;Li A;Liu X
长链非编码RNA（longnoncodingRNA，lncRNA）是近年来发现的一类在肿瘤发生中起重要作用的RNA。然而，SNHG 17在肝细胞癌（HCC）中的表达模式和生物学功能尚不清楚。采用生物信息学分析和qRT-PCR方法检测SNHG 17在肝癌组织、癌旁组织和细胞系中的表达情况。通过在肝癌细胞系中敲低和过表达SNHG 17来研究SNHG 17对肝癌细胞增殖、迁移和凋亡的影响。利用RNA测序来探索潜在的机制。利用公开可用的TCGA-LIHC、GSE 102079 HCC数据集和qRT-PCR，我们发现SNHG 17在HCC组织和细胞系中显著上调，并且与较大的肿瘤大小、低分化、血管浸润的存在和晚期TNM分期显著相关。此外，功能获得和丧失研究表明，SNHG 17促进细胞增殖和迁移，并抑制肝癌细胞凋亡。通过RNA测序，我们发现SNHG 17的敲除导致了1037个差异表达基因，这些基因高度富集在多个途径中，包括代谢、PI 3 K-Akt、细胞粘附、细胞增殖调控和凋亡途径;其中92个与TCGA-LIHC数据集中SNHG 17相关基因重叠。此外，ERH、TBCA、TDO 2和PDK 4被成功验证，并在HCC组织中发现显著失调。SNHG 17高表达的HCC患者的总生存期和无病生存期相对较低，ERH和PDK 4在HCC的预后中也起着明显的作用。总之，我们的研究结果表明SNHG 17在HCC进展中作为非编码癌基因发挥作用，表明其作为HCC治疗新靶点的潜在价值。
Long noncoding RNAs (lncRNAs) have been substantially reported to have critical roles in regulating tumorigenesis in recent years. However, the expression pattern and biological function of SNHG17 in hepatocellular carcinoma (HCC) remain unclear. Bioinformatics analysis and qRT-PCR were performed to detect the expression pattern of SNHG17 in HCC tissues, adjacent nontumorous tissues, and cell lines. The effect of SNHG17 on proliferation, migration, and apoptosis of HCC was investigated by knockdown and overexpressing SNHG17 in HCC cell lines. RNA sequencing was utilized to explore the underlying mechanism. Utilizing publicly available TCGA-LIHC, GSE102079 HCC datasets, and qRT-PCR, we found SNHG17 was significantly upregulated in HCC tissues and cell lines and was notably associated with larger tumor size, poorly differentiation, presence of vascular invasion, and advanced TNM stage. Furthermore, gain- and loss-of-function studies demonstrated that SNHG17 promoted cell proliferation and migration and inhibited apoptosis of HCC. By employing RNA sequencing, we found knockdown of SNHG17 caused 1037 differentially expressed genes, highly enriched in several pathways, including metabolic, PI3K-Akt, cell adhesion, regulation of cell proliferation, and apoptotic pathway; among them, 92 were overlapped with SNHG17-related genes in the TCGA-LIHC dataset. Furthermore, ERH, TBCA, TDO2, and PDK4 were successfully validated and found significantly dysregulated in HCC tissues. Moreover, HCC patients with higher SNHG17 expression had a relatively poor overall survival and disease-free survival, and ERH and PDK4 also played a marked role in the prognosis of HCC. Broadly, our findings illustrate that SNHG17 acts as a noncoding oncogene in HCC progression, suggesting its potential value as a novel target for HCC therapy.
登录
查看更多内容
癌症中的长非编码 RNA：信号传导电路。
影响因子：
19
作者：
Lin C;Yang L
通讯作者：
Yang L
影响因子：
8.8
作者：
Strowitzki, Moritz J.;Radhakrishnan, Praveen;Schneider, Martin
通讯作者：
Schneider, Martin
影响因子：
37.3
作者：
Huo X;Han S;Wu G;Latchoumanin O;Zhou G;Hebbard L;George J;Qiao L
通讯作者：
Qiao L
影响因子：
14.8
作者：
Pertea, Mihaela;Kim, Daehwan;Pertea, Geo M.;Leek, Jeffrey T.;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
14.8
作者：
Huang, Da Wei;Sherman, Brad T.;Lempicki, Richard A.
通讯作者：
Lempicki, Richard A.