One-Step Azolation Strategy for Site- and Chemo-Selective Labeling of Proteins with Mass-Sensitive Probes.
One-Step Azolation Strategy for Site- and Chemo-Selective Labeling of Proteins with Mass-Sensitive Probes.
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一步偶氮化策略的位点和化学选择性标记与质量敏感探针的蛋白质。
DOI：
10.1002/anie.202007608
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发表时间：
2021-01-25
影响因子：
16.6
通讯作者：
Raj, Monika
中科院分区：
文献类型：
--
作者：
Tang, Kuei C.;Raj, Monika
以位点选择性方式对蛋白质进行化学修饰导致了各个科学领域的许多进展。常规N-末端生物缀合技术的主要挑战是缺乏通用序列相容性和所得生物缀合物的质量检测灵敏度差。这种方法有效地分析复杂混合物中的蛋白水解片段和天然蛋白质。通常需要多个化学步骤来定点合成具有增强的质量检测灵敏度的生物缀合物。我们提出了一个单一的步骤，多功能的策略，选择性修饰蛋白质N-末端的质量助推器。化学标签将肽检测增强了数倍，从而对所得生物缀合物进行了明确的分析。我们证明，在复杂的混合物中用质量敏感性探针标记蛋白水解片段提高了所得生物缀合物的检测。我们报告了一种新的一步唑化策略的网站和化学选择性标记的N-末端与质量敏感的探针。该方法对多种蛋白质具有耐受性，并适用于在温和的反应条件下，在一锅法中对消化的复杂混合物中的蛋白水解片段进行N-末端标记。
The chemical modification of proteins in a site-selective manner leads to many advances in various scientific fields. The major challenges with conventional N-terminal bioconjugation techniques are the lack of universal sequence compatibility and poor mass detection sensitivity of resulting bioconjugates. This approach efficiently analyze proteolytic fragments and native proteins in a complex mixture. Multiple chemical steps are usually required for the site-selective synthesis of bioconjugates with enhanced mass detection sensitivity. We present a single step, versatile strategy for the selective modification of protein N-termini with mass boosters. The chemical tag enhances the peptide detection by multiple orders thus leads to the unambiguous analysis of resulting bioconjugates. We demonstrate that tagging proteolytic fragments with mass sensitivity probes in a complex mixture improves the detection of resulting bioconjugates. We report a novel one-step Azolation strategy for the site- and chemo-selective labeling of the N-terminus with mass sensitive probes. This method is tolerant to a variety of proteins and is applied for the N-terminal tagging of proteolytic fragments in the digested complex mixture in one-pot, under mild reaction conditions.
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影响因子：
56.9
作者：
DAWSON, PE;MUIR, TW;KENT, SBH
通讯作者：
KENT, SBH
影响因子：
15
作者：
Adusumalli, Srinivasa Rao;Rawale, Tdattatraya Gautam;Rai, Vishal
通讯作者：
Rai, Vishal
影响因子：
16.6
作者：
Purushottam, Landa;Adusumalli, Srinivasa Rao;Rai, Vishal
通讯作者：
Rai, Vishal
影响因子：
3.9
作者：
Fernandez-Moreira, Vanesa;Alegre-Requena, Juan V.;Concepcion Gimeno, M.
通讯作者：
Concepcion Gimeno, M.
影响因子：
1.8
作者：
Li, XF;Zhang, LS;Tam, JP
通讯作者：
Tam, JP