Spectroscopic photoacoustic imaging of cervical tissue composition in excised human samples.
Spectroscopic photoacoustic imaging of cervical tissue composition in excised human samples.
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DOI：
10.1371/journal.pone.0247385
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Mehrmohammadi M
中科院分区：
文献类型：
--
作者：
Yan Y;Basij M;Garg A;Varrey A;Alhousseini A;Hsu R;Hernandez-Andrade E;Romero R;Hassan SS;Mehrmohammadi M
宫颈重塑是决定分娩途径的重要组成部分；因此，评估宫颈组织成分的变化可能会提供有关宫颈状况的信息，而不是测量宫颈长度。光声成像是一种基于超声的非侵入性技术，它捕获组织组件响应激光脉冲发出的声学信号。这种光学信息允许确定胶原蛋白与水的比率(CWR)。本研究的目的是比较用光谱光声成像(SpA)对孕妇和非孕妇的宫颈标本进行CWR的评估。这项横断面研究包括子宫切除时(n=8)和未分娩的足月孕妇(n=8)的预定剖宫产时的宫颈活检。利用集成在超声探头上的光纤光传输系统对颈部CWR进行分析。在覆盖胶原蛋白和水的吸收峰的波长范围内采集了光声信号。分析孕妇与非孕妇宫颈CWR的差异。苏木精-伊红染色和天狼星红染色比较两组宫颈标本的胶原含量。子宫切除术后取宫颈标本8例，其中41岁≤妇女4例，43岁≥妇女4例，所有孕妇(n=8)在剖宫产时妊娠37周后采集宫颈标本。妊娠妇女宫颈组织平均CWR为18.7%(SD 7.5%)，非妊娠妇女CWR为55.0%(SD 20.3%)。非妊娠组的CWR显著高于妊娠组，p值为0.001。亚组分析比较了41岁≤孕妇和非孕妇宫颈样本的CWR(平均46.3%，SD 23.1%)，也显示CWR显著高于非孕妇(P<；0.01)。组织学分析证实，妊娠组胶原含量较低，组织成分丢失，水分含量增加，胶原降解。所提出的双模超声和水疗成像系统可以提供怀孕和非怀孕妇女宫颈组织生化成分的信息。光声成像显示，与妊娠妇女相比，非怀孕妇女宫颈样本中的胶原含量更高，这与组织学分析相一致。这一新的成像方法为评估宫颈组织成分提供了一种新的灵敏诊断工具。
Cervical remodeling is an important component in determining the pathway of parturition; therefore, assessing changes in cervical tissue composition may provide information about the cervix’s status beyond the measurement of cervical length. Photoacoustic imaging is a non-invasive ultrasound-based technology that captures acoustic signals emitted by tissue components in response to laser pulses. This optical information allows for the determination of the collagen-to-water ratio (CWR). The purpose of this study was to compare the CWR evaluated by using spectroscopic photoacoustic (sPA) imaging in cervical samples obtained from pregnant and non-pregnant women. This cross-sectional study comprised cervical biopsies obtained at the time of hysterectomy (n = 8) and at the scheduled cesarean delivery in pregnant women at term who were not in labor (n = 8). The cervical CWR was analyzed using a fiber-optic light-delivery system integrated to an ultrasound probe. The photoacoustic signals were acquired within the range of wavelengths that cover the peak absorption of collagen and water. Differences in the CWR between cervical samples from pregnant and non-pregnant women were analyzed. Hematoxylin and eosin and Sirius Red stains were used to compare the collagen content of cervical samples in these two groups. Eight cervix samples were obtained after hysterectomy, four from women ≤41 years of age and four from women ≥43 years of age; all cervical samples (n = 8) from pregnant women were obtained after 37 weeks of gestation at the time of cesarean section. The average CWR in cervical tissue samples from pregnant women was 18.7% (SD 7.5%), while in samples from non-pregnant women, it was 55.0% (SD 20.3%). There was a significantly higher CWR in the non-pregnant group compared to the pregnant group with a p-value <0.001. A subgroup analysis that compared the CWR in cervical samples from pregnant women and non-pregnant women ≤41 years of age (mean 46.3%, SD 23.1%) also showed a significantly higher CWR (p <0.01). Lower collagen content in the pregnancy group was confirmed by histological analysis, which revealed the loss of tissue composition, increased water content, and collagen degradation. The proposed bimodal ultrasound and sPA imaging system can provide information on the biochemical composition of cervical tissue in pregnant and non-pregnant women. Photoacoustic imaging showed a higher collagen content in cervical samples from non-pregnant women as compared to those from pregnant women, which matched with the histological analysis. This novel imaging method envisions a new potential for a sensitive diagnostic tool in the evaluation of cervical tissue composition.
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影响因子：
3.6
作者：
Li C;Yang JM;Chen R;Yeh CH;Zhu L;Maslov K;Zhou Q;Shung KK;Wang LV
通讯作者：
Wang LV
影响因子：
2.3
作者：
Feltovich, Helen;Nam, Kibo;Hall, Timothy J.
通讯作者：
Hall, Timothy J.
影响因子：
7.1
作者：
Hassan, S. S.;Romero, R.;Creasy, G. W.
通讯作者：
Creasy, G. W.
影响因子：
2.4
作者：
Banos, Ana;Wolf, Martin;Hornung, Rene
通讯作者：
Hornung, Rene
DOI：
10.1016/0006-2944(79)90081-4
发表时间：
1979-01-01
期刊：
BIOCHEMICAL MEDICINE
影响因子：
--
作者：
ITO, A;KITAMURA, K;HIRAKAWA, S
通讯作者：
HIRAKAWA, S