Extended Ischemic Recovery After Implantation of Human Mesenchymal Stem Cell Aggregates Indicated by Sodium MRI at 21.1 T.
Extended Ischemic Recovery After Implantation of Human Mesenchymal Stem Cell Aggregates Indicated by Sodium MRI at 21.1 T.
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DOI：
10.1007/s12975-021-00976-4
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发表时间：
2022-08
影响因子：
6.9
通讯作者：
Grant, Samuel C.
中科院分区：
文献类型：
--
作者：
Helsper, Shannon;Bagdasarian, F. Andrew;Yuan, Xuegang;Xu, Kaya;Lee, Jea-Young;Rosenberg, Jens T.;Borlongan, Cesario, V;Ma, Teng;Grant, Samuel C.
在使用干细胞疗法治疗缺血性中风中，扩展治疗应用仍然是一个重要问题。沿着这些路线，在将用氧化铁的微米级颗粒标记的人间充质干细胞聚集体（hMSC-agg）直接植入到缺血性损伤半球对侧的侧脑室中之后，评价缺血性中风的啮齿动物模型中的神经恢复。纵向上，通过21.1 T下的1H和23 Na磁共振成像（MRI）评估疾病进展和对hMSC-agg治疗的反应，以研究细胞定位、迁移和延长时间范围内的恢复。除了传统的质子成像之外，MRI还通过钠分布提供了组织状态的可量化指标。定量23 Na MRI显示hMSC聚集体植入后钠浓度显著降低，表明稳态恢复。该结果与通过行为分析和免疫组织化学评估的延长的神经恢复正相关。这些发现证明了植入的hMSC聚集疗法为缺血性卒中提供扩展治疗的潜力，以及MRI用于监测此类方法的稳健性。这种方法可能被转化为临床环境，用于评估延长的细胞治疗功效。
Extended therapeutic application remains a significant issue in the use of stem cell therapies to treat ischemic stroke. Along these lines, neurological recovery in a rodent model of ischemic stroke was evaluated following implantation of human mesenchymal stem cell aggregates (hMSC-agg), labeled with micron-sized particles of iron oxide, directly into the lateral ventricle contralateral to the ischemic lesion hemisphere. Longitudinally, disease progression and response to hMSC-agg therapy were assessed by 1H and 23Na magnetic resonance imaging (MRI) at 21.1 T to investigate cellular localization, migration, and recovery over an extended timeframe. MRI provides quantifiable metrics of tissue status through sodium distributions in addition to traditional proton imaging. Quantitative 23Na MRI revealed a significant decrease of sodium concentrations following hMSC aggregate implantation, indicating recovery of homeostasis. This result correlates positively with extended neurological recovery assessed by behavioral analysis and immunohistochemistry. These findings demonstrate the potential of implanted hMSC aggregate therapy to provide extended treatment for ischemic stroke, as well as the robustness of MRI for monitoring such approaches. This method potentially can be translated to a clinical setting for the assessment of extended cell therapy efficacy.
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