Differential anti-glycan antibody responses in Schistosoma mansoni-infected children and adults studied by shotgun glycan microarray.
Differential anti-glycan antibody responses in Schistosoma mansoni-infected children and adults studied by shotgun glycan microarray.
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DOI：
10.1371/journal.pntd.0001922
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发表时间：
2012
影响因子：
3.8
通讯作者：
Hokke CH
中科院分区：
文献类型：
--
作者：
van Diepen A;Smit CH;van Egmond L;Kabatereine NB;Pinot de Moira A;Dunne DW;Hokke CH
血吸虫病（血吸虫病）是一种慢性和潜在致命的寄生虫病，影响（亚）热带地区数百万人。在血吸虫病流行地区，对血吸虫感染的重要部分免疫力确实会产生，但这需要多年的暴露和免疫系统的成熟。因此，儿童在治疗后比年龄较大的儿童和成人更容易再次感染。这种年龄依赖性免疫或对再感染的易感性已被证明与特异性抗体和T细胞反应有关。血吸虫感染期间产生的许多抗体针对血吸虫表达的许多聚糖。天然血吸虫感染血清中抗体识别的聚糖表位的性质在很大程度上是未知的。使用聚糖微阵列方法可以有效地和定量地分析血清抗体与聚糖的结合。非常少量的大量聚糖存在于固体表面上，允许测试各种聚糖结合蛋白的结合特性。我们已经产生了一个所谓的鸟枪聚糖微阵列含有天然N-聚糖和脂质聚糖馏分来自4个不同的生活阶段的S。mansoni的，并应用该阵列的分析IgG和IgM抗体的血清中的儿童和成人生活在流行区。这导致鉴别出两个不同年龄组特有的差异聚糖识别谱，可能反映了年龄差异或暴露或感染时间长短差异。使用鸟枪聚糖微阵列方法来研究抗体对同源体衍生的聚糖元件的反应谱，我们已经定义了感染个体的组以及在S.曼氏感染。这些发现对于进一步探索血吸虫多糖抗原与免疫的关系具有重要意义。血吸虫是一种寄生蠕虫，在（亚）热带地区的数百万人中引起慢性和潜在致命的疾病。一个重要的部分免疫感染发展，但这需要多年的接触和多次感染。因此，儿童在治疗后远比成人更容易再次感染。这种免疫保护与特异性抗体和T细胞反应有关。血吸虫感染期间产生的许多抗体针对寄生虫表达的碳水化合物链（聚糖）。天然血吸虫感染血清中抗体识别的聚糖表位的性质在很大程度上是未知的。我们使用所谓的鸟枪聚糖微阵列方法来研究链球菌之间抗聚糖抗体反应的差异。感染曼氏菌的儿童和成人。这导致确定了两个不同年龄组的差异聚糖识别特征，这可能反映了生活在流行地区的人的年龄差异或暴露或感染时间长短的差异。
Schistosomiasis (bilharzia) is a chronic and potentially deadly parasitic disease that affects millions of people in (sub)tropical areas. An important partial immunity to Schistosoma infections does develop in disease endemic areas, but this takes many years of exposure and maturation of the immune system. Therefore, children are far more susceptible to re-infection after treatment than older children and adults. This age-dependent immunity or susceptibility to re-infection has been shown to be associated with specific antibody and T cell responses. Many antibodies generated during Schistosoma infection are directed against the numerous glycans expressed by Schistosoma. The nature of glycan epitopes recognized by antibodies in natural schistosomiasis infection serum is largely unknown. The binding of serum antibodies to glycans can be analyzed efficiently and quantitatively using glycan microarray approaches. Very small amounts of a large number of glycans are presented on a solid surface allowing binding properties of various glycan binding proteins to be tested. We have generated a so-called shotgun glycan microarray containing natural N-glycan and lipid-glycan fractions derived from 4 different life stages of S. mansoni and applied this array to the analysis of IgG and IgM antibodies in sera from children and adults living in an endemic area. This resulted in the identification of differential glycan recognition profiles characteristic for the two different age groups, possibly reflecting differences in age or differences in length of exposure or infection. Using the shotgun glycan microarray approach to study antibody response profiles against schistosome-derived glycan elements, we have defined groups of infected individuals as well as glycan element clusters to which antibody responses are directed in S. mansoni infections. These findings are significant for further exploration of Schistosoma glycan antigens in relation to immunity. Schistosomes are parasitic worms that cause chronic and potentially deadly disease in millions of people in (sub)tropical areas. An important partial immunity to infection does develop but this takes many years of exposure and multiple infections. Therefore, children are far more susceptible to re-infection after treatment than adults. This immunological protection is associated with specific antibody and T cell responses. Many antibodies generated during Schistosoma infection are directed against carbohydrate chains (glycans) expressed by the parasite. The nature of the glycan epitopes recognized by antibodies in natural schistosomiasis infection serum is largely unknown. We have used a so-called shotgun glycan microarray approach to study differences in anti-glycan antibody responses between S. mansoni-infected children and adults. This resulted in the identification of differential glycan recognition profiles characteristic for the two different age groups that may reflect differences in age or differences in length of exposure or infection in people living in an endemic area.
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影响因子：
7.4
作者：
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通讯作者：
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DOI：
10.1016/j.trstmh.2005.03.016
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2006-03-01
影响因子：
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通讯作者：
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DOI：
10.1016/j.trstmh.2004.06.003
发表时间：
2004-12-01
影响因子：
2.2
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