Proteomic comparison of Ralstonia solanacearum strains reveals temperature dependent virulence factors.
Proteomic comparison of Ralstonia solanacearum strains reveals temperature dependent virulence factors.
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DOI：
10.1186/1471-2164-15-280
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发表时间：
2014-04-12
期刊：
影响因子：
4.4
通讯作者：
Norman DJ
中科院分区：
文献类型：
--
作者：
Bocsanczy AM;Achenbach UC;Mangravita-Novo A;Chow M;Norman DJ
青枯雷尔氏菌（Ralstonia solanacearum）是细菌性枯萎病的病原体，是存在于世界热带和亚热带地区的遗传多样性细菌性植物病原体，其感染超过200种植物物种，包括经济上重要的茄科作物。大多数菌株R.青枯菌仅在25至30°C之间温度下致病，而在低于20°C下可引起疾病的菌株被认为是对温带地区农业的威胁。确定关键的分子因素，区分菌株在寒冷的温度下有毒的，不需要开发有效的管理工具，这种病原体。当在30 ° C和18°C下在番茄幼苗根际培养时，我们使用定量2D DIGE凝胶方法比较了两种低温毒性菌株和两种低温非毒性菌株的蛋白质谱。对斑点强度进行定量和比较，并对差异表达的蛋白质进行测序和质谱（MS/MS）鉴定。对418个差异表达的蛋白质点进行测序，产生101个独特蛋白质。所鉴定的蛋白质被分类在基因本体生物学过程类别中，包括代谢、细胞过程、应激反应、运输、分泌、运动性和毒力。鉴定的毒力因子包括过氧化氢酶（KatE）、外切葡聚糖酶A（ChbA）、药物外排泵和颤搐运动孔蛋白（PilQ）。确定的其他蛋白质包括两个组件的一个假定的VI型分泌系统。我们用真实的时间PCR技术证实了13个候选基因的差异表达。当通过真实的时间PCR定量时，全局调节因子HrpB和HrpG也具有温度依赖性表达。假定参与鉴定的蛋白质在低温下的毒力进行了讨论。功能性VI型分泌系统的发现提供了一个新的潜在的毒力机制进行探索。全球监管机构HrpG和HrpB，和蛋白质表达谱表明，在低温下的毒力可以部分地解释在所有菌株中存在的毒力因子的调节的差异。
Ralstonia solanacearum, the causal agent of bacterial wilt, is a genetically diverse bacterial plant pathogen present in tropical and subtropical regions of the world that infects more than 200 plant species, including economically important solanaceous crops. Most strains of R. solanacearum are only pathogenic at temperatures between 25 to 30°C with strains that can cause disease below 20°C considered a threat to agriculture in temperate areas. Identifying key molecular factors that distinguish strains virulent at cold temperatures from ones that are not is needed to develop effective management tools for this pathogen. We compared protein profiles of two strains virulent at low temperature and two strains not virulent at low temperature when incubated in the rhizosphere of tomato seedlings at 30 and 18°C using quantitative 2D DIGE gel methods. Spot intensities were quantified and compared, and differentially expressed proteins were sequenced and identified by mass spectrometry (MS/MS). Four hundred and eighteen (418) differentially expressed protein spots sequenced produced 101 unique proteins. The identified proteins were classified in the Gene Ontology biological processes categories of metabolism, cell processes, stress response, transport, secretion, motility, and virulence. Identified virulence factors included catalase (KatE), exoglucanase A (ChbA), drug efflux pump, and twitching motility porin (PilQ). Other proteins identified included two components of a putative type VI secretion system. We confirmed differential expression of 13 candidate genes using real time PCR techniques. Global regulators HrpB and HrpG also had temperature dependent expression when quantified by real time PCR. The putative involvement of the identified proteins in virulence at low temperature is discussed. The discovery of a functional type VI secretion system provides a new potential virulence mechanism to explore. The global regulators HrpG and HrpB, and the protein expression profiles identified suggest that virulence at low temperatures can be partially explained by differences in regulation of virulence factors present in all the strains.
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影响因子：
3.6
作者：
Colmer, JA;Fralick, JA;Hamood, AN
通讯作者：
Hamood, AN
影响因子：
3.5
作者：
Flores-Cruz, Zomary;Allen, Caitilyn
通讯作者：
Allen, Caitilyn
影响因子：
13.1
作者：
Beranova-Giorgianni, S
通讯作者：
Beranova-Giorgianni, S
DOI：
10.1007/bf02854025
发表时间：
1980-01-01
期刊：
AMERICAN POTATO JOURNAL
影响因子：
--
作者：
CIAMPI, L;SEQUEIRA, L
通讯作者：
SEQUEIRA, L
影响因子：
3.2
作者：
Haapalainen, Minna;Mosorin, Hanna;Lin, Nai-Chun
通讯作者：
Lin, Nai-Chun