Silencing circRNA_001937 may inhibit cutaneous squamous cell carcinoma proliferation and induce apoptosis by preventing the sponging of the miRNA‑597‑3p/FOSL2 pathway.
Silencing circRNA_001937 may inhibit cutaneous squamous cell carcinoma proliferation and induce apoptosis by preventing the sponging of the miRNA‑597‑3p/FOSL2 pathway.
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DOI：
10.3892/ijmm.2020.4723
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发表时间：
2020-11
影响因子：
5.4
通讯作者：
Lin Q
中科院分区：
文献类型：
--
作者：
Gao L;Jin HJ;Zhang D;Lin Q
环状RNA(CircRNAs)被报道在多种类型的肿瘤中异常表达并发挥不同的功能；然而，它们在皮肤鳞状细胞癌(CSCC)中的表达水平仍不清楚。因此，本研究的目的是探讨CircRNA_001937在宫颈鳞癌中的作用。应用Arraystar Human CircRNAs芯片和逆转录定量聚合酶链式反应(RT-qPCR)分析宫颈鳞癌CircRNA差异表达谱，采用双荧光素酶报告基因分析方法研究CircRNA_001937对细胞行为的影响，特别是对微RNA(MiRNA)-597-3p/Fos相关抗原2(FOSL2)通路的调控，并用RT-qPCR和Western blotting进行验证。宫颈鳞癌组织和细胞系中CircRNA_001937的表达水平显著高于相应的癌旁组织和对照细胞(P<；0.05)。小干扰RNA使CircRNA_001937基因沉默，显著抑制细胞增殖，诱导细胞凋亡(P<；0.05)。观察到CircRNA_001937直接与miRNA597-3p结合，并作为海绵，间接增加miRNA597-3p靶基因FOSL2的表达水平。结论：宫颈鳞癌组织中CircRNA597-001937表达增加，沉默CircRNA597-3p/fosl2通路可能抑制宫颈鳞癌的进展。
Circular RNAs (circRNAs) are reported to be aberrantly expressed and perform different functions in numerous types of tumor; however, their expression levels in cutaneous squamous cell carcinoma (CSCC) remain largely unclear. Thus, the purpose of the present study was to investigate the function of circRNA_001937 in CSCC. Differential circRNA expression profiles of CSCC were analyzed using the Arraystar Human circRNAs chip and reverse transcription-quantitative PCR (RT-qPCR); and the effects of circRNA_001937 on cell behavior, in particular its regulation over the microRNA (miRNA)-597-3p/Fos-related antigen 2 (FOSL2) pathway, was investigated using a dual-luciferase reporter assay, and verified using RT-qPCR and western blotting. circRNA_001937 expression levels were significantly increased in CSCC tissues and cell lines compared with the corresponding adjacent tissues and control cells (P<0.05). The genetic silencing of circRNA_001937 with small interfering RNA significantly inhibited cell proliferation, and induced cell apoptosis (P<0.05). circRNA_001937 was observed to directly bind to miRNA-597-3p and serve as a sponge, which indirectly increased the expression levels of FOSL2, a miRNA-597-3p target gene. In conclusion, circRNA_001937 expression was increased in CSCC and silencing circRNA_001937 gene expression may inhibit CSCC progression by sponging the miRNA-597-3p/FOSL2 pathway.
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