Ecdysone exerts biphasic control of regenerative signaling, coordinating the completion of regeneration with developmental progression.
Ecdysone exerts biphasic control of regenerative signaling, coordinating the completion of regeneration with developmental progression.
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DOI：
10.1073/pnas.2115017119
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发表时间：
2022-02-01
影响因子：
11.1
通讯作者：
Halme A
中科院分区：
文献类型：
--
作者：
Karanja F;Sahu S;Weintraub S;Bhandari R;Jaszczak R;Sitt J;Halme A
For most organisms, regenerative capacity varies at different stages of development. Changes in regenerative capacity often correlate with significant changes in systemic hormone signaling. Previous studies have independently demonstrated the positive and negative effects of systemic hormone signals on the regenerative activity of tissues. Here, we report that regenerating Drosophila melanogaster tissues produce a biphasic response to ecdysone steroid hormone levels. Below a certain threshold, ecdysone promotes regenerative activity in damaged imaginal discs. As development progresses, ecdysone levels increase above this threshold and suppress regeneration via the sequential expression of Broad transcription factor splice isoforms. Our findings describe how systemic hormone signals can direct regenerative activity to coordinate regeneration completion with developmental transitions. In Drosophila melanogaster, loss of regenerative capacity in wing imaginal discs coincides with an increase in systemic levels of the steroid hormone ecdysone, a key coordinator of their developmental progression. Regenerating discs release the relaxin hormone Dilp8 (Drosophila insulin-like peptide 8) to limit ecdysone synthesis and extend the regenerative period. Here, we describe how regenerating tissues produce a biphasic response to ecdysone levels: lower concentrations of ecdysone promote local and systemic regenerative signaling, whereas higher concentrations suppress regeneration through the expression of broad splice isoforms. Ecdysone also promotes the expression of wingless during both regeneration and normal development through a distinct regulatory pathway. This dual role for ecdysone explains how regeneration can still be completed successfully in dilp8−mutant larvae: higher ecdysone levels increase the regenerative activity of tissues, allowing regeneration to reach completion in a shorter time. From these observations, we propose that ecdysone hormone signaling functions to coordinate regeneration with developmental progression.
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DOI：
10.1016/j.cub.2010.01.038
发表时间：
2010-03-09
期刊：
Current biology : CB
影响因子：
--
作者：
Halme A;Cheng M;Hariharan IK
通讯作者：
Hariharan IK
影响因子：
4.6
作者：
Cherbas, L;Hu, X;Cherbas, P
通讯作者：
Cherbas, P
影响因子：
4.6
作者：
Lavrynenko, Oksana;Rodenfels, Jonathan;Shevchenko, Andrej
通讯作者：
Shevchenko, Andrej
影响因子：
--
作者：
HOPKINS, PM
通讯作者：
HOPKINS, PM
影响因子：
4
作者：
Jaszczak, Jacob S.;Halme, Adrian
通讯作者：
Halme, Adrian