Growth of Porphyromonas gingivalis on human serum albumin triggers programmed cell death.
Growth of Porphyromonas gingivalis on human serum albumin triggers programmed cell death.
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DOI：
10.1080/20002297.2022.2161182
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发表时间：
2023
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
牙龈沟液（GCF）是牙龈卟啉单胞菌在体内的主要生长基质。这项工作的目的是评估不同菌株的牙龈卟啉单胞菌对人血清白蛋白（HSA），GCF的主要成分的生长。检查了五种不同的牙龈卟啉单胞菌菌株在HSA培养基中的生长，令人惊讶的是，其中三种菌株在24小时内发生自溶。比较转录组学分析用于鉴定参与自溶的基因。两个高度相关的参考菌株（W50和W83）在HSA上生长时，其存活率存在显着差异。菌株W83生长迅速，在24 h内裂解，而W50存活额外的20 h。差异基因表达分析导致我们的基因簇包含参与精氨酸代谢的酶和预测为裂解性胞壁蛋白转糖基酶的基因，这些基因已知在自溶中发挥作用。该基因（PG 0139）的缺失导致不裂解的突变体，并且互补恢复了HSA裂解表型，表明该酶在牙龈卟啉单胞菌的自溶中起核心作用。当提供HSA作为生长底物时，牙龈卟啉单胞菌经历自溶。
Gingival crevicular fluid (GCF) constitutes the primary growth substrate for Porphyromonas gingivalis in vivo. The goal of this work was to evaluate the growth of different strains of P. gingivalis on human serum albumin (HSA), a major constituent of GCF. Growth of five different strains of P. gingivalis in the HSA medium was examined and, surprisingly, three of the strains underwent autolysis within 24 h. Comparative transcriptomic analysis was used to identify genes involved in autolysis. Two highly related reference strains (W50 and W83) differed dramatically in their survival when grown on HSA. Strain W83 grew fast and lysed within 24 h, while W50 survived for an additional 20 h. Differential gene expression analysis led us to a gene cluster containing enzymes involved in arginine metabolism and a gene predicted to be lytic murein transglycosylase, which are known to play a role in autolysis. Deletion of this gene (PG0139) resulted in a mutant that did not lyse, and complementation restored the HSA lysis phenotype, indicating that this enzyme plays a central role in the autolysis of P. gingivalis. P. gingivalis undergoes autolysis when provided with HSA as a substrate for growth.
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影响因子：
3.7
作者：
Beaume M;Hernandez D;Farinelli L;Deluen C;Linder P;Gaspin C;Romby P;Schrenzel J;Francois P
通讯作者：
Francois P
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
14.9
作者：
Caspi R;Billington R;Ferrer L;Foerster H;Fulcher CA;Keseler IM;Kothari A;Krummenacker M;Latendresse M;Mueller LA;Ong Q;Paley S;Subhraveti P;Weaver DS;Karp PD
通讯作者：
Karp PD
影响因子：
3.7
作者：
Hirano T;Beck DA;Wright CJ;Demuth DR;Hackett M;Lamont RJ
通讯作者：
Lamont RJ
影响因子：
3.2
作者：
Dashper, Stuart G.;Ang, Ching-Seng;Reynolds, Eric C.
通讯作者：
Reynolds, Eric C.