Culturomics- and metagenomics-based insights into the microbial community and function of rhizosphere soils in Sinai desert farming systems.
Culturomics- and metagenomics-based insights into the microbial community and function of rhizosphere soils in Sinai desert farming systems.
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DOI：
10.1186/s40793-023-00463-3
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发表时间：
2023-01-13
影响因子：
7.9
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中科院分区：
文献类型：
--
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西奈沙漠耕作系统的微生物群在恶劣、极端的沙漠环境中种植作物的适应性策略中发挥着重要作用。然而，在这种不利的水分和营养条件下，微生物群落的多样性和功能尚未得到调查。本研究基于培养和元基因组学方法，对西奈沙漠4个农场12种植物根际共14个土壤样品进行了微生物多样性和功能分析，为微生物接种剂的设计提供了有价值和有意义的指导。结果揭示了广泛的微生物分类群，包括高比例的新的未描述的谱系。根际微生物群落组成因采样点不同而不同，尽管植物区系有相似之处或不同之处。而不同采样点的根际微生物群落和植物本身的功能特征不同，但其功能特征却非常相似。重要的是，参与生态系统功能的微生物在不同采样点之间是不同的，例如，所有采样点都普遍存在固氮，而负责这一过程的微生物不同。在这里，我们首次对西奈沙漠耕作系统根际土壤的微生物群落和功能进行了表征，并强调了其出人意料的高度多样性。本研究提供的证据表明，不同环境条件的采样点参与生态系统功能的关键微生物不同，强调了根际微生物群落功能微生物群的重要性。此外，我们建议用于未来农业生产的微生物接种剂应选择能够参与植物-微生物相互作用并已适应类似环境的微生物。网上版载有补充材料，可在10.1186/s40793-023-00463-3查阅。
The microbiome of the Sinai Desert farming system plays an important role in the adaptive strategy of growing crops in a harsh, poly-extreme, desert environment. However, the diversity and function of microbial communities under this unfavorable moisture and nutritional conditions have not yet been investigated. Based on culturomic and metagenomic methods, we analyzed the microbial diversity and function of a total of fourteen rhizosphere soil samples (collected from twelve plants in four farms of the Sinai desert), which may provide a valuable and meaningful guidance for the design of microbial inoculants. The results revealed a wide range of microbial taxa, including a high proportion of novel undescribed lineages. The composition of the rhizosphere microbial communities differed according to the sampling sites, despite similarities or differences in floristics. Whereas, the functional features of rhizosphere microbiomes were significantly similar in different sampling sites, although the microbial communities and the plant hosts themselves were different. Importantly, microorganisms involved in ecosystem functions are different between the sampling sites, for example nitrogen fixation was prevalent in all sample sites while microorganisms responsible for this process were different. Here, we provide the first characterization of microbial communities and functions of rhizosphere soil from the Sinai desert farming systems and highlight its unexpectedly high diversity. This study provides evidence that the key microorganisms involved in ecosystem functions are different between sampling sites with different environment conditions, emphasizing the importance of the functional microbiomes of rhizosphere microbial communities. Furthermore, we suggest that microbial inoculants to be used in future agricultural production should select microorganisms that can be involved in plant-microorganism interactions and are already adapted to a similar environmental setting. The online version contains supplementary material available at 10.1186/s40793-023-00463-3.
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