Production and utilization of extracellular matrix components by human melanocytes.
Production and utilization of extracellular matrix components by human melanocytes.
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人类黑素细胞细胞外基质成分的产生和利用。
DOI：
10.1016/0014-4827(89)90060-8
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发表时间：
1989
影响因子：
3.7
通讯作者：
Varani,J
中科院分区：
文献类型：
--
作者：
McClenic,BK;Mitra,RS;Riser,BL;Nickoloff,BJ;Dixit,VM;Varani,J
Normal human melanocytes were separated from keratinocytes and maintained in culture using KGM medium supplemented with 12-O-tetradecanoylphorbol acetate and cholera toxin. The melanocytes were examined for the production of extracellular matrix molecules including fibronectin, laminin, and thrombospondin and for the utilization of these molecules in adhesion and motility assays. Melanocytes produced significant amounts of fibronectin as indicated by biosynthetic labeling/immunoprecipitation and by enzyme-linked immunosorbent assay (ELISA). Fibronectin was expressed on the surface of these cells. Laminin was also produced by melanocytes and expressed on the cell surface. The amount of laminin produced was significantly less than the amount of fibronectin. In contrast, melanocytes did not produce measurable thrombospondin as indicated by biosynthetic labeling/immunoprecipitation. Only traces of thrombospondin were detected by ELISA and no surface fluorescence was observed. When examined in adhesion and motility assays, melanocytes were found to utilize fibronectin for both processes. Laminin also stimulated adhesion but it was much less effective than fibronectin. Thrombospondin did not stimulate either attachment and spreading or motility. The pattern of extracellular matrix molecule production and utilization by melanocytes is significantly different from that shown previously for human epidermal keratinocytes (J. Varaniet al., 1988,J. Clin. Invest.81, 1537). These differences may underlie the differences with which the two cell types interact with basement membranesin vivo.
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DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Davis,RJ;Ganong,BR;Bell,RM;Czech,MP
通讯作者：
Czech,MP
DOI：
--
发表时间：
1985-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
G. Grynkiewicz;M. Poenie;Roger Y. TsienB
通讯作者：
G. Grynkiewicz;M. Poenie;Roger Y. TsienB
年龄对正常和骨质疏松女性血清免疫反应性甲状旁腺激素的影响。
DOI：
--
发表时间：
1980
期刊：
Journal of Laboratory and Clinical Medicine
影响因子：
--
作者：
J. Gallagher;B. Riggs;C. Jerpbak;C. Arnaud
通讯作者：
C. Arnaud
促甲状腺激素释放激素诱导 GH4C1 大鼠垂体细胞中蛋白激酶 C 的重新分布。
DOI：
--
发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Fearon,CW;TashjianJr,AH
通讯作者：
TashjianJr,AH
细胞外 Ca 和 Mg 对分散牛甲状旁腺细胞胞质 Ca 和 PTH 释放的影响。
影响因子：
4.8
作者：
D. Shoback;J. Thatcher;R. Leombruno;E. Brown
通讯作者：
E. Brown