Structural and molecular micropatterning of dual hydrogel constructs for neural growth models using photochemical strategies.
Structural and molecular micropatterning of dual hydrogel constructs for neural growth models using photochemical strategies.
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DOI：
10.1007/s10544-012-9687-y
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发表时间：
2013-02
影响因子：
2.8
通讯作者：
Moore, Michael J.
中科院分区：
文献类型：
--
作者：
Horn-Ranney, Elaine L.;Curley, J. Lowry;Catig, Gary C.;Huval, Renee M.;Moore, Michael J.
趋化和触触线索通过引起神经突生长锥的吸引或排斥反应来引导神经突向适当的目标生长。在这里，我们提出了一个集成系统，允许在双水凝胶 3D 组织培养结构中进行结构和分子微图案化，通过结构、固定和可溶性引导线索指导体外神经元生长。使用从充当动态光掩模的数字微镜装置反射的紫外光将这些组织培养构建体加工成可指定的几何形状，从而形成双水凝胶构建体，该构建体由限制细胞生长的聚乙二醇（PEG）边界和允许细胞生长的光不稳定的α-羧基-2-硝基苄基半胱氨酸琼脂糖（CNBC-A）内部组成。该 CNBC-A 在离散区域受到辐射，随后用马来酰亚胺结合的生物分子进行标记。荧光显微镜显示生物分子仅在 CNBC-A 的照射部位结合，共焦显微镜证实了构建体深度的 3D 结合。神经突生长研究表明，整个 CNBC-A 期间神经突生长受到抑制。可溶性荧光素-牛血清白蛋白通过双水凝胶结构的扩散速率由PEG浓度和蛋白源与琼脂糖内部之间的距离控制；瞬时蛋白质梯度的时间尺度随着这些参数的变化而变化。这些发现表明，双水凝胶系统是操纵 3D 体外微环境的有用平台，具有用于建模神经生长和引导的图案化结构和分子引导线索。
Chemotactic and haptotactic cues guide neurite growth toward appropriate targets by eliciting attractive or repulsive responses from the neurite growth cones. Here we present an integrated system allowing both structural and molecular micropatterning in dual hydrogel 3D tissue culture constructs for directing in vitro neuronal growth via structural, immobilized, and soluble guidance cues. These tissue culture constructs were fabricated into specifiable geometries using UV light reflected from a digital micromirror device acting as a dynamic photomask, resulting in dual hydrogel constructs consisting of a cell growth-restrictive polyethylene glycol (PEG) boundary with a cell growth-permissive interior of photolabile α-carboxy-2-nitrobenzyl cysteine agarose (CNBC-A). This CNBC-A was irradiated in discrete areas and subsequently tagged with maleimide-conjugated biomolecules. Fluorescent microscopy showed biomolecule binding only at the sites of irradiation in CNBC-A, and confocal microscopy confirmed 3D binding through the depth of the construct. Neurite outgrowth studies showed contained growth throughout CNBC-A. The diffusion rate of soluble fluorescein-bovine serum albumin through the dual hydrogel construct was controlled by PEG concentration and the distance between the protein source and the agarose interior; the timescale for a transient protein gradient changed with these parameters. These findings suggest the dual hydrogel system is a usefulplatform for manipulating a 3D in vitro microenvironment with patterned structural and molecular guidance cues for modeling neural growth and guidance.
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影响因子：
4.9
作者：
Curley, J. Lowry;Moore, Michael J.
通讯作者：
Moore, Michael J.
DOI：
10.1039/c0ib00038h
发表时间：
2010-11
期刊：
Integrative biology : quantitative biosciences from nano to macro
影响因子：
--
作者：
Bhattacharjee N;Li N;Keenan TM;Folch A
通讯作者：
Folch A
影响因子：
--
作者：
CHANG, CY;NIBLACK, B;BAYLEY, H
通讯作者：
BAYLEY, H
影响因子：
56.9
作者：
Cukierman, E;Pankov, R;Yamada, KM
通讯作者：
Yamada, KM
影响因子：
41.2
作者：
Luo, Y;Shoichet, MS
通讯作者：
Shoichet, MS