Facile micropatterning of dual hydrogel systems for 3D models of neurite outgrowth.
Facile micropatterning of dual hydrogel systems for 3D models of neurite outgrowth.
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DOI：
10.1002/jbm.a.33195
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发表时间：
2011-12-15
影响因子：
4.9
通讯作者：
Moore, Michael J.
中科院分区：
文献类型：
--
作者：
Curley, J. Lowry;Moore, Michael J.
了解微环境因素如何影响神经突生长对于神经再生，可塑性，发育和神经生理学的研究非常重要。试图通过提供3D生长基质来更准确地模拟生理环境的体外模型可以提供有用的基础。厚的3D培养模型的一些限制包括阻碍溶质运输，比在2D基板上更不稳健的神经突生长，以及难以实现生长的空间控制。为此，我们描述了一个3D双水凝胶模型胚胎大鼠15天背根神经节组织外植体生长使用数字微镜装置的动态掩模投影光刻。所开发的光刻方法允许在各种基底上简单、可再现、一步制造厚水凝胶构建体，包括可渗透的细胞培养插入物。投影掩模尺寸，交联水凝胶分辨率，和凝胶厚度之间的关系进行了表征，并发现分辨率一般随着凝胶厚度的增加而降低。厚（481 μm）水凝胶构建体中的细胞活力在可渗透支持物上显著高于玻璃，表明转运限制在一定程度上得到缓解。观察到的神经突生长是丰富的，并发生在整个3D环境中的空间控制的方式，在寻求一个更有效的神经突生长的仿生模型的关键一步。
Understanding how microenvironmental factors influence neurite growth is important to inform studies in nerve regeneration, plasticity, development, and neurophysiology. In vitro models attempting to more accurately mimic the physiological environment by provision of a 3D growth matrix may provide useful foundations. Some limitations of thick 3D culture models include hampered solute transport, less-robust neurite growth than on 2D substrates, and difficulty in achieving spatial control of growth. To this end, we describe a 3D dual hydrogel model for embryonic rat day 15 dorsal root ganglion tissue explant growth using a digital micro-mirror device for dynamic mask projection photolithography. The photolithography method developed allowed simple, reproducible, one-step fabrication of thick hydrogel constructs on a variety of substrates, including permeable cell culture inserts. The relationships between projected mask size, crosslinked hydrogel resolution, and gel thickness were characterized, and resolution was found generally to decrease with increasing gel thickness. Cell viability in thick (481 μm) hydrogel constructs was significantly greater on permeable supports than glass, suggesting transport limitations were somewhat alleviated. The observed neurite growth was abundant and occurred in a spatially controlled manner throughout the 3D environment, a crucial step in the quest for a more effective biomimetic model of neurite outgrowth.
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影响因子：
14
作者：
Johansson, F;Carlberg, P;Kanje, M
通讯作者：
Kanje, M
影响因子：
41.2
作者：
Luo, Y;Shoichet, MS
通讯作者：
Shoichet, MS
影响因子：
2.7
作者：
ANTON, ES;SANDROCK, AW;MATTHEW, WD
通讯作者：
MATTHEW, WD
纳米神经编织：用于大脑修复和轴突再生以及视力功能恢复的肽纳米纤维支架
DOI：
10.1073/pnas.0600559103
发表时间：
2006-03-28
影响因子：
11.1
作者：
Ellis-Behnke, RG;Liang, YX;Schneider, GE
通讯作者：
Schneider, GE
DOI：
10.1073/pnas.97.12.6728
发表时间：
2000-06-06
影响因子：
11.1
作者：
Holmes, TC;de Lacalle, S;Zhang, SG
通讯作者：
Zhang, SG