Hsp27 associates with the titin filament system in heat-shocked zebrafish cardiomyocytes.
Hsp27 associates with the titin filament system in heat-shocked zebrafish cardiomyocytes.
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DOI：
10.1016/j.yexcr.2009.06.030
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发表时间：
2009-11-01
影响因子：
3.7
通讯作者：
Shelden EA
中科院分区：
文献类型：
--
作者：
Tucker NR;Shelden EA
Injury to muscle tissue plays a central role in various cardiovascular pathologies. Overexpression of the small heat shock protein Hsp27 protects muscle cells against thermal, oxidative and ischemic stress. However, underlying mechanisms of this protection have not been resolved. A distinctive feature of muscle cells is the stress-induced association of Hsp27 with the sarcomere. The association of Hsp27 with the cytoskeleton, in both muscle and non-muscle cells, is thought to represent interaction with Z-line components or filamentous actin. Here, we examined the association of Hsp27 with myofibrils in adult zebrafish myocardium subjected to hyperthermia and mechanical stretching. Consistent with previously published results, Hsp27 in resting length myofibrils localized to narrowly defined regions, or bands, which colocalized with Z-line markers. However, analysis of stretched myofibrils revealed that the association of Hsp27 with myofibrils was independent of desmin, alpha-actinin, myosin, and filamentous actin. Instead, Hsp27 maintained a consistent relationship with a marker for the titin A/I border over various sarcomeric lengths. Finally, extraction of actin filaments revealed that Hsp27 binds to a component of the remaining sarcomere. Together, these novel data support a mechanism of Hsp27 function where interactions with the titin filament system protect myofibrils from stress-induced degradation.
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影响因子：
4.1
作者：
Bryantsev, Anton L.;Kurchashova, Svetlana Yu.;Kampinga, Harm H.
通讯作者：
Kampinga, Harm H.
DOI：
10.1002/dvg.1020140204
发表时间：
1993-01-01
期刊：
DEVELOPMENTAL GENETICS
影响因子：
--
作者：
GERNOLD, M;KNAUF, U;KLOETZEL, PM
通讯作者：
KLOETZEL, PM
影响因子：
37.8
作者：
Hollander, JM;Martin, JL;Dillmann, WH
通讯作者：
Dillmann, WH
影响因子：
3.8
作者：
Bonham, RT;Fine, MR;Shelden, EA
通讯作者：
Shelden, EA
影响因子：
5.5
作者：
Féasson, L;Stockholm, D;Denis, C
通讯作者：
Denis, C