Exercise training enhances in vivo clearance of endotoxin and attenuates inflammatory responses by potentiating Kupffer cell phagocytosis.
Exercise training enhances in vivo clearance of endotoxin and attenuates inflammatory responses by potentiating Kupffer cell phagocytosis.
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DOI：
10.1038/s41598-017-12358-8
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发表时间：
2017-09-20
影响因子：
4.6
通讯作者：
Shoda J
中科院分区：
文献类型：
--
作者：
Komine S;Akiyama K;Warabi E;Oh S;Kuga K;Ishige K;Togashi S;Yanagawa T;Shoda J
枯否细胞（KCs）清除内毒素的功能障碍是非酒精性脂肪性肝病（NAFLD）发病的重要因素。在这项研究中，运动训练对KC功能的影响进行了研究，在体内内毒素清除和炎症反应。将小鼠分为休息组和运动组。KC珠吞噬能力和血浆类固醇激素水平测定运动训练后。在注射内毒素后随时间测定血浆中的内毒素和炎性细胞因子水平。运动组KC珠吞噬能力增强，对外源性内毒素的清除率增加。运动组的炎性细胞因子（TNF-α和IL-6）水平较低。我们发现，只有DHEA在运动组的血浆中增加。在体外实验中，向RAW264.7细胞中添加DHEA增加了珠粒吞噬能力并减弱了内毒素诱导的炎症反应。这些结果表明，运动训练调节体内内毒素清除和炎症反应与增加DHEA生产。这些运动诱导的KC容量变化可能有助于减缓NAFLD患者的疾病进展。
The failure of Kupffer cells (KCs) to remove endotoxin is an important factor in the pathogenesis of non-alcoholic fatty liver disease (NAFLD). In this study, the effects of exercise training on KC function were studied in terms of in vivo endotoxin clearance and inflammatory responses. Mice were allocated into rest and exercise groups. KC bead phagocytic capacity and plasma steroid hormone levels were determined following exercise training. Endotoxin and inflammatory cytokine levels in plasma were determined over time following endotoxin injection. KC bead phagocytic capacity was potentiated and clearance of exogenously-injected endotoxin was increased in the exercise group. Inflammatory cytokine (TNF-α and IL-6) levels were lower in the exercise group. We found that only DHEA was increased in the plasma of the exercise group. In an in vitro experiment, the addition of DHEA to RAW264.7 cells increased bead phagocytic capacity and attenuated endotoxin-induced inflammatory responses. These results suggest that exercise training modulates in vivo endotoxin clearance and inflammatory responses in association with increased DHEA production. These exercise-induced changes in KC capacity may contribute to a slowing of disease progression in NAFLD patients.
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影响因子：
4.6
作者：
Oh S;So R;Shida T;Matsuo T;Kim B;Akiyama K;Isobe T;Okamoto Y;Tanaka K;Shoda J
通讯作者：
Shoda J
影响因子：
4.9
作者：
Ayi, K;Giribaldi, G;Arese, P
通讯作者：
Arese, P
DOI：
10.1111/j.1600-0838.2010.01132.x
发表时间：
2012-02-01
影响因子：
4.1
作者：
Ortega, E.;Bote, M. E.;Garcia, J. J.
通讯作者：
Garcia, J. J.
影响因子：
4.4
作者：
Mancuso, P;Gottschalk, A;Huffnagle, GB
通讯作者：
Huffnagle, GB
影响因子：
3
作者：
Ben-Nathan, D;Padgett, DA;Loria, RM
通讯作者：
Loria, RM