Coverage of diarrhoea-associated Escherichia coli isolates from different origins with two types of phage cocktails.
Coverage of diarrhoea-associated Escherichia coli isolates from different origins with two types of phage cocktails.
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DOI：
10.1111/1751-7915.12113
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发表时间：
2014-03
影响因子：
5.7
通讯作者：
Brüssow H
中科院分区：
文献类型：
--
作者：
Bourdin G;Navarro A;Sarker SA;Pittet AC;Qadri F;Sultana S;Cravioto A;Talukder KA;Reuteler G;Brüssow H
从我们的噬菌体收集的89个T4样噬菌体与代表不同地理来源(墨西哥和孟加拉国的 )、血清型(69 O，27H血清型)、致病型(肠出血性肠杆菌、肠出血性肠杆菌、肠出血性肠出血性志贺氏菌)、流行病学环境(社区和住院腹泻)和隔离年限的四组儿童腹泻相关大肠杆菌分离株进行了测试。在由3到14个T4样噬菌体组成的鸡尾酒中，我们对来自墨西哥的主要EPEC分离株实现了54%到69%的覆盖率，对孟加拉国前瞻性调查中主要的ETEC分离株实现了30%到53%的覆盖率，对孟加拉国住院儿童的混合病原型分离株实现了24%到61%的覆盖率，对志贺氏菌株的覆盖率达到了60%。相比之下，商业俄罗斯噬菌体鸡尾酒包含许多不同属的大肠杆菌噬菌体的复杂混合物，对上述四个集合的覆盖率分别为19%、33%、50%和90%。在我们的T4类噬菌体收集中，几乎没有检测到O型特异性噬菌体，没有检测到广泛宿主范围的噬菌体。当组成较大的噬菌体鸡尾酒时，观察到噬菌体分离物之间的干扰现象。由于给定的T4类噬菌体鸡尾酒的覆盖率随地理区域和流行病学环境的不同而不同，因此针对E. 大肠杆菌病原体的噬菌体治疗方法需要一种适合当地情况的噬菌体成分。
Eighty-nine T4-like phages from our phage collection were tested against four collections of childhood diarrhoea-associated Escherichia coli isolates representing different geographical origins (Mexico versus Bangladesh), serotypes (69 O, 27 H serotypes), pathotypes (ETEC, EPEC, EIEC, EAEC, VTEC, Shigella), epidemiological settings (community and hospitalized diarrhoea) and years of isolation. With a cocktail consisting of 3 to 14 T4-like phages, we achieved 54% to 69% coverage against predominantly EPEC isolates from Mexico, 30% to 53% against mostly ETEC isolates from a prospective survey in Bangladesh, 24% to 61% against a mixture of pathotypes isolated from hospitalized children in Bangladesh, and 60% coverage against Shigella isolates. In comparison a commercial Russian phage cocktail containing a complex mixture of many different genera of coliphages showed 19%, 33%, 50% and 90% coverage, respectively, against the four above-mentioned collections. Few O serotype-specific phages and no broad-host range phages were detected in our T4-like phage collection. Interference phenomena between the phage isolates were observed when constituting larger phage cocktails. Since the coverage of a given T4-like phage cocktail differed with geographical area and epidemiological setting, a phage composition adapted to a local situation is needed for phage therapy approaches against E. coli pathogens.
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影响因子：
3.2
作者：
ABEDON, ST
通讯作者：
ABEDON, ST
影响因子：
64.8
作者：
Forde, Samantha E.;Beardmore, Robert E.;Hurst, Laurence D.
通讯作者：
Hurst, Laurence D.
影响因子：
5.6
作者：
HASHEMOLHOSSEINI, S;MONTAG, D;HENNING, U
通讯作者：
HENNING, U
影响因子：
3
作者：
Chang, Ho-Won;Kim, Kyoung-Ho
通讯作者：
Kim, Kyoung-Ho
DOI：
10.4269/ajtmh.2008.79.708
发表时间：
2008-11-01
影响因子：
3.3
作者：
Harris, Aaron M.;Chowdhury, Fahima;Qadri, Firdausi
通讯作者：
Qadri, Firdausi