Distinct origins and genetic programs of head muscle satellite cells.
Distinct origins and genetic programs of head muscle satellite cells.
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DOI：
10.1016/j.devcel.2009.05.007
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发表时间：
2009-06
影响因子：
11.8
通讯作者：
Tzahor, Eldad
中科院分区：
文献类型：
--
作者：
Harel, Itamar;Nathan, Elisha;Tirosh-Finkel, Libbat;Zigdon, Hila;Guimaraes-Camboa, Nuno;Evans, Sylvia M.;Tzahor, Eldad
成人骨骼肌具有显着的再生能力，由于卫星细胞，成人肌肉干细胞的存在。我们在鸟类和小鼠模型中使用了命运映射技术，以显示躯干（Pax3+）和颅骨（MesP1+）骨骼肌和卫星细胞来自不同的遗传谱系。类似的谱系异质性也见于头部肌肉组织和卫星细胞，这是由于它们共有的、异质的胚胎起源。用Isl1Cre小鼠进行的谱系追踪实验证明了Isl1+细胞对不同的颌肌衍生卫星细胞的强大贡献。将肌纤维相关Isl 1衍生的卫星细胞移植到受损的肢体肌肉中有助于肌肉再生。体外实验证明了颅源性卫星细胞而非躯干源性卫星细胞的心源性。最后，在鸡胚鳃肌Isl1过表达抑制骨骼肌分化在体外和体内，这表明该基因在心血管和骨骼肌干细胞祖细胞的规范中发挥作用。
Adult skeletal muscle possesses a remarkable regenerative capacity, due to the presence of satellite cells, adult muscle stem cells. We used fate-mapping techniques in avian and mouse models to show that trunk (Pax3+) and cranial (MesP1+) skeletal muscle and satellite cells derive from separate genetic lineages. Similar lineage heterogeneity is seen within the head musculature and satellite cells, due to their shared, heterogenic embryonic origins. Lineage tracing experiments with Isl1Cre mice demonstrated the robust contribution of Isl1+ cells to distinct jaw muscle-derived satellite cells. Transplantation of myofiber-associated Isl1-derived satellite cells into damaged limb muscle contributed to muscle regeneration. In vitro experiments demonstrated the cardiogenic nature of cranial- but not trunk-derived satellite cells. Finally, overexpression of Isl1 in the branchiomeric muscles of chick embryos inhibited skeletal muscle differentiation in vitro and in vivo, suggesting that this gene plays a role in the specification of cardiovascular and skeletal muscle stem cell progenitors.
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