Mining the A.E. Watkins Wheat Landrace Collection for Variation in Starch Digestibility Using a New High-Throughput Assay.

Mining the A.E. Watkins Wheat Landrace Collection for Variation in Starch Digestibility Using a New High-Throughput Assay.
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DOI:
10.3390/foods12020266
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发表时间:
2023-01-06
期刊:
Foods (Basel, Switzerland)
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其他
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培育小麦中不易消化的淀粉可以改善面包和其他小麦食品的健康影响。正向遗传方法的应用最近为发现影响淀粉消化率的新基因提供了机会,而且不会对产量或面食和面包制作质量产生有害影响。在这项研究中,我们开发了一种高通量体外淀粉消化率测定(HTA),用于筛选小麦种质的正向遗传方法。 HTA 使用标准玉米和小麦淀粉进行了验证。使用 HTA,我们测量了水热处理面粉样品中的淀粉消化率,发现 A. E. Watkins 收藏的 118 种小麦地方品种和 8 个英国精英品种之间存在很大差异(90 分钟后淀粉消化率分别为 23.5% 至 39.9% 和 31.2% 至 43.5%)。我们进一步研究了沃特金斯品系和优良品种中的筛分全麦面粉和纯化淀粉的淀粉消化率,发现面粉的基质特性而不是淀粉颗粒的内在特性赋予较低的淀粉消化率。
Breeding for less digestible starch in wheat can improve the health impact of bread and other wheat foods. The application of forward genetic approaches has lately opened opportunities for the discovery of new genes that influence the digestibility of starch, without the burden of detrimental effects on yield or on pasta and bread-making quality. In this study we developed a high-throughput in vitro starch digestibility assay (HTA) for use in forward genetic approaches to screen wheat germplasm. The HTA was validated using standard maize and wheat starches. Using the HTA we measured starch digestibility in hydrothermally processed flour samples and found wide variation among 118 wheat landraces from the A. E. Watkins collection and among eight elite UK varieties (23.5 to 39.9% and 31.2 to 43.5% starch digested after 90 min, respectively). We further investigated starch digestibility in fractions of sieved wholemeal flour and purified starch in a subset of the Watkins lines and elite varieties and found that the matrix properties of flour rather than the intrinsic properties of starch granules conferred lower starch digestibility.
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