Mining the A.E. Watkins Wheat Landrace Collection for Variation in Starch Digestibility Using a New High-Throughput Assay.
Mining the A.E. Watkins Wheat Landrace Collection for Variation in Starch Digestibility Using a New High-Throughput Assay.
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DOI:
10.3390/foods12020266
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发表时间:
2023-01-06
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影响因子:
--
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Breeding for less digestible starch in wheat can improve the health impact of bread and other wheat foods. The application of forward genetic approaches has lately opened opportunities for the discovery of new genes that influence the digestibility of starch, without the burden of detrimental effects on yield or on pasta and bread-making quality. In this study we developed a high-throughput in vitro starch digestibility assay (HTA) for use in forward genetic approaches to screen wheat germplasm. The HTA was validated using standard maize and wheat starches. Using the HTA we measured starch digestibility in hydrothermally processed flour samples and found wide variation among 118 wheat landraces from the A. E. Watkins collection and among eight elite UK varieties (23.5 to 39.9% and 31.2 to 43.5% starch digested after 90 min, respectively). We further investigated starch digestibility in fractions of sieved wholemeal flour and purified starch in a subset of the Watkins lines and elite varieties and found that the matrix properties of flour rather than the intrinsic properties of starch granules conferred lower starch digestibility.
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影响因子:
6.1
作者:
Corrado M;Ahn-Jarvis JH;Fahy B;Savva GM;Edwards CH;Hazard BA
通讯作者:
Hazard BA
影响因子:
6.9
作者:
Fahy B;Siddiqui H;David LC;Powers SJ;Borrill P;Uauy C;Smith AM
通讯作者:
Smith AM
影响因子:
13.8
作者:
Botticella E;Sestili F;Sparla F;Moscatello S;Marri L;Cuesta-Seijo JA;Falini G;Battistelli A;Trost P;Lafiandra D
通讯作者:
Lafiandra D
影响因子:
1.9
作者:
Bansal, Urmil K.;Arief, Vivi N.;Bariana, Harbans S.
通讯作者:
Bariana, Harbans S.
DOI:
10.1111/j.1467-789x.2012.01011.x
发表时间:
2012-10
期刊:
Obesity reviews : an official journal of the International Association for the Study of Obesity
影响因子:
--
作者:
Blaak EE;Antoine JM;Benton D;Björck I;Bozzetto L;Brouns F;Diamant M;Dye L;Hulshof T;Holst JJ;Lamport DJ;Laville M;Lawton CL;Meheust A;Nilson A;Normand S;Rivellese AA;Theis S;Torekov SS;Vinoy S
通讯作者:
Vinoy S