Screening of candidate regulators for cellulase and hemicellulase production in Trichoderma reesei and identification of a factor essential for cellulase production.
Screening of candidate regulators for cellulase and hemicellulase production in Trichoderma reesei and identification of a factor essential for cellulase production.
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DOI：
10.1186/1754-6834-7-14
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发表时间：
2014-01-28
影响因子：
6.3
通讯作者：
Pakula TM
中科院分区：
文献类型：
--
作者：
Häkkinen M;Valkonen MJ;Westerholm-Parvinen A;Aro N;Arvas M;Vitikainen M;Penttilä M;Saloheimo M;Pakula TM
软腐子囊菌里氏木霉用于工业生产分泌酶，特别是木质纤维素降解酶。T. Reesei使用几种不同的酶来降解植物细胞壁来源的材料，包括9种表征的纤维素酶、15种表征的半纤维素酶和至少42种预测编码纤维素分解或半纤维素分解活性的基因。纤维素酶和半纤维素酶的产生受环境和生理条件调节。已经鉴定出几种影响纤维素酶和半纤维素酶基因表达的调节因子，但据信T. reesei。我们使用了T. reesei培养物，其中通过添加不同的木质纤维素衍生材料诱导纤维素酶/半纤维素酶的产生，以鉴定纤维素酶和半纤维素酶基因的推定的新调节剂。基于这些诱导数据，并补充了其他已发表的关于不同蛋白质产生条件的全基因组数据，选择了28个候选调控基因进行进一步研究，并将它们在T. reesei。与亲本菌株相比，7个基因的过表达导致在修饰的菌株中纤维素酶和/或木聚糖酶活性的产生增加至少1.5倍。发现基因77513（在此指定为ace 3）的缺失对纤维素酶生产和所研究的几个纤维素酶基因的表达是有害的。这种缺失也显著降低了木聚糖酶活性和木聚糖降解酶基因的表达。此外，我们的数据揭示了存在的共调节的染色体区域含有碳水化合物活性酶基因和候选调控基因。从糖苷水解酶诱导实验结合特定的蛋白质生产条件的先前研究的转录分析结果被证明是一种有效的方法，用于寻找新的候选调控基因影响生产的纤维素酶和半纤维素酶。构建了产纤维素酶和/或木聚糖酶的重组菌株，发现了纤维素酶基因表达所必需的基因，进一步证实了碳水化合物活性酶基因表达在染色质水平上的区域调控。
The soft rot ascomycetal fungus Trichoderma reesei is utilized for industrial production of secreted enzymes, especially lignocellulose degrading enzymes. T. reesei uses several different enzymes for the degradation of plant cell wall-derived material, including 9 characterized cellulases, 15 characterized hemicellulases and at least 42 genes predicted to encode cellulolytic or hemicellulolytic activities. Production of cellulases and hemicellulases is modulated by environmental and physiological conditions. Several regulators affecting the expression of cellulase and hemicellulase genes have been identified but more factors still unknown are believed to be present in the genome of T. reesei. We have used transcriptional profiling data from T. reesei cultures in which cellulase/hemicellulase production was induced by the addition of different lignocellulose-derived materials to identify putative novel regulators for cellulase and hemicellulase genes. Based on this induction data, supplemented with other published genome-wide data on different protein production conditions, 28 candidate regulatory genes were selected for further studies and they were overexpressed in T. reesei. Overexpression of seven genes led to at least 1.5-fold increased production of cellulase and/or xylanase activity in the modified strains as compared to the parental strain. Deletion of gene 77513, here designated as ace3, was found to be detrimental for cellulase production and for the expression of several cellulase genes studied. This deletion also significantly reduced xylanase activity and expression of xylan-degrading enzyme genes. Furthermore, our data revealed the presence of co-regulated chromosomal regions containing carbohydrate-active enzyme genes and candidate regulatory genes. Transcriptional profiling results from glycoside hydrolase induction experiments combined with a previous study of specific protein production conditions was shown to be an effective method for finding novel candidate regulatory genes affecting the production of cellulases and hemicellulases. Recombinant strains with improved cellulase and/or xylanase production properties were constructed, and a gene essential for cellulase gene expression was found. In addition, more evidence was gained on the chromatin level regional regulation of carbohydrate-active enzyme gene expression.
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影响因子：
6.3
作者：
Kubicek CP;Mikus M;Schuster A;Schmoll M;Seiboth B
通讯作者：
Seiboth B
影响因子：
3.7
作者：
Ivanova C;Bååth JA;Seiboth B;Kubicek CP
通讯作者：
Kubicek CP
影响因子：
3.6
作者：
KULMBURG, P;MATHIEU, M;FELENBOK, B
通讯作者：
FELENBOK, B
影响因子：
14.9
作者：
Hunter S;Jones P;Mitchell A;Apweiler R;Attwood TK;Bateman A;Bernard T;Binns D;Bork P;Burge S;de Castro E;Coggill P;Corbett M;Das U;Daugherty L;Duquenne L;Finn RD;Fraser M;Gough J;Haft D;Hulo N;Kahn D;Kelly E;Letunic I;Lonsdale D;Lopez R;Madera M;Maslen J;McAnulla C;McDowall J;McMenamin C;Mi H;Mutowo-Muellenet P;Mulder N;Natale D;Orengo C;Pesseat S;Punta M;Quinn AF;Rivoire C;Sangrador-Vegas A;Selengut JD;Sigrist CJ;Scheremetjew M;Tate J;Thimmajanarthanan M;Thomas PD;Wu CH;Yeats C;Yong SY
通讯作者：
Yong SY
影响因子：
3.7
作者：
Engler, Carola;Kandzia, Romy;Marillonnet, Sylvestre
通讯作者：
Marillonnet, Sylvestre