Identification and characterization of microRNAs in the flag leaf and developing seed of wheat (Triticum aestivum L.).
Identification and characterization of microRNAs in the flag leaf and developing seed of wheat (Triticum aestivum L.).
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DOI：
10.1186/1471-2164-15-289
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发表时间：
2014-04-16
期刊：
影响因子：
4.4
通讯作者：
Zhao H
中科院分区：
文献类型：
--
作者：
Han R;Jian C;Lv J;Yan Y;Chi Q;Li Z;Wang Q;Zhang J;Liu X;Zhao H
MicroRNAs(MiRNAs)调控植物体内的各种生物过程。关于参与水稻、玉米和大麦发育的miRNAs，有大量数据可用。相比之下，人们对miRNAs及其在小麦发育中的作用知之甚少。本研究从小麦幼苗、旗叶和发育中的种子中构建了5个小RNA(SRNA)文库，并对其进行了测序，以鉴定miRNAs并了解其在小麦发育中的功能。本研究从小麦的18个miRNA基因座中鉴定出15个miRNA家族的24个已知miRNAs，其中15个miRNAs(9个miRNA基因座)在小麦中首次发现，13个miRNA家族(16个miRNA基因座)高度保守，2个(2个miRNA基因座)中等保守。此外，还鉴定了55个新的miRNAs。用严格的标准预测了15个已知miRNAs和37个新miRNAs的潜在靶基因，这些靶基因涉及广泛的生物学功能。已知的15个miRNA家族中的4个和55个新的miRNA家族中的22个在发育中的种子中优先表达，其倍数变化的对数(Log2)为1.0~7.6，其中一半是种子特异性的，表明它们参与了小麦种子的发育和代谢调控。从花后5d到花后20d，miR164和miR160在发育中的种子中含量增加，而miR169含量减少，表明它们在小麦种子不同发育阶段的协调作用。此外，已知的8个miRNA家族和28个新的miRNAs在小麦旗叶中表现出组织偏向表达，其倍数变化的对数为0.1~5.2。这些组织选择性miRNAs的可能靶点参与了不同的代谢和生物学过程，表明不同组织中调控网络的复杂性。我们的数据还表明，小麦旗叶具有比发育中的种子更复杂的miRNAs调控网络。我们的工作确定并表征了小麦miRNAs、它们的靶标和表达模式。本研究首次阐明了小麦旗叶和种子发育过程中miRNAs的调控网络，为进一步研究这些miRNAs的特定功能奠定了基础。
MicroRNAs (miRNAs) regulate various biological processes in plants. Considerable data are available on miRNAs involved in the development of rice, maize and barley. In contrast, little is known about miRNAs and their functions in the development of wheat. In this study, five small RNA (sRNA) libraries from wheat seedlings, flag leaves, and developing seeds were developed and sequenced to identify miRNAs and understand their functions in wheat development. Twenty-four known miRNAs belonging to 15 miRNA families were identified from 18 MIRNA loci in wheat in the present study, including 15 miRNAs (9 MIRNA loci) first identified in wheat, 13 miRNA families (16 MIRNA loci) being highly conserved and 2 (2 MIRNA loci) moderately conserved. In addition, fifty-five novel miRNAs were also identified. The potential target genes for 15 known miRNAs and 37 novel miRNAs were predicted using strict criteria, and these target genes are involved in a wide range of biological functions. Four of the 15 known miRNA families and 22 of the 55 novel miRNAs were preferentially expressed in the developing seeds with logarithm (log2) of the fold change of 1.0 ~ 7.6, and half of them were seed-specific, suggesting that they participate in regulating wheat seed development and metabolism. From 5 days post-anthesis to 20 days post-anthesis, miR164 and miR160 increased in abundance in the developing seeds, whereas miR169 decreased, suggesting their coordinating functions in the different developmental stages of wheat seed. Moreover, 8 known miRNA families and 28 novel miRNAs exhibited tissue-biased expression in wheat flag leaves, with the logarithm of the fold changes of 0.1 ~ 5.2. The putative targets of these tissue-preferential miRNAs were involved in various metabolism and biological processes, suggesting complexity of the regulatory networks in different tissues. Our data also suggested that wheat flag leaves have more complicated regulatory networks of miRNAs than developing seeds. Our work identified and characterised wheat miRNAs, their targets and expression patterns. This study is the first to elucidate the regulatory networks of miRNAs involved in wheat flag leaves and developing seeds, and provided a foundation for future studies on specific functions of these miRNAs.
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影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
1.8
作者：
HOFACKER, IL;FONTANA, W;SCHUSTER, P
通讯作者：
SCHUSTER, P
影响因子：
7.4
作者：
Jain, Mukesh;Nijhawan, Aashima;Khurana, Jitendra P.
通讯作者：
Khurana, Jitendra P.
影响因子：
16
作者：
Jones-Rhoades, MW;Bartel, DP
通讯作者：
Bartel, DP
影响因子：
4.4
作者：
Cantu D;Vanzetti LS;Sumner A;Dubcovsky M;Matvienko M;Distelfeld A;Michelmore RW;Dubcovsky J
通讯作者：
Dubcovsky J