Capillary electrophoresis coupled with automated fraction collection.
Capillary electrophoresis coupled with automated fraction collection.
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DOI：
10.1016/j.talanta.2014.07.018
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发表时间：
2014-12
期刊：
影响因子：
6.1
通讯作者：
Dovichi, Norman J.
中科院分区：
文献类型：
--
作者：
Huge, Bonnie Jaskowski;Flaherty, Ryan J.;Dada, Oluwatosin O.;Dovichi, Norman J.
研制了一种基于按需滴液式喷墨打印机的级分收集器，用于毛细管区带电泳与96孔微量滴定板的连接。我们首先通过使用毛细管区带电泳来分析四甲基罗丹明的1 mM溶液来评估收集器的性能;然后使用荧光微量滴定板读数器来检测分析物并表征威尔斯孔之间的级分携带。峰高的相对标准偏差为20%，迁移时间的相对标准偏差为1%。四甲基罗丹明峰宽的平均值和标准偏差为5 ± 1 s，可能受到液滴沉积之间4 s时间的限制。接下来，我们注射了DNA片段的复杂混合物，并在CE-SELEX实验中使用实时PCR来定量产物。重建的电泳峰持续时间为27 s。最后，我们在CE-SELEX条件下，在30 μM凝血酶溶液的存在下重复实验;收集组分，并使用下一代测序来表征DNA结合剂。超过25，000个序列被鉴定为与已知的凝血酶结合适体密切匹配。
A fraction collector based on a drop-on-demand ink-jet printer was developed to interface capillary zone electrophoresis with a 96 well microtiter plate. We first evaluated the performance of the collector by using capillary zone electrophoresis to analyze a 1 mM solution of tetramethylrhodamine; a fluorescent microtiter plate reader was then used to detect the analyte and characterize fraction carryover between wells. Relative standard deviation in peak height was 20% and the relative standard deviation in migration time was 1%. The mean and standard deviation of the tetramethylrhodamine peak width was 5 ± 1 s and likely limited by the 4-s period between droplet deposition. We next injected a complex mixture of DNA fragments and used real-time PCR to quantify the product in a CE-SELEX experiment. The reconstructed electrophoretic peak was 27 s in duration. Finally, we repeated the experiment in the presence of a 30-μM thrombin solution under CE-SELEX conditions; fractions were collected and next-generation sequencing was used to characterize the DNA binders. Over 25,000 sequences were identified with close matches to known thrombin binding aptamers.
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毛细管区电泳，用于使用电动泵送的护套纳米喷雾界面分析复杂蛋白质组织。
影响因子：
3.4
作者：
Sun, Liangliang;Zhu, Guijie;Yan, Xiaojing;Champion, Mathew M.;Dovichi, Norman J.
通讯作者：
Dovichi, Norman J.
影响因子：
5.6
作者：
Tasset, DM;Kubik, MF;Steiner, W
通讯作者：
Steiner, W
影响因子：
2.9
作者：
Vannatta, Michael W.;Whitmore, Colin D.;Dovichi, Norman J.
通讯作者：
Dovichi, Norman J.
毛细血管电泳中的六个数量级动态范围，具有超敏激光诱导的荧光检测。
影响因子：
6.1
作者：
Whitmore, Colin D.;Essaka, David;Dovichi, Norman J.
通讯作者：
Dovichi, Norman J.
影响因子：
14.9
作者：
Ikebukuro K;Okumura Y;Sumikura K;Karube I
通讯作者：
Karube I