Induction of β-Defensin Expression by Porphyromonas gingivalis-Infected Human Gingival Graft Transplanted in nu/nu Mouse Subdermis.
Induction of β-Defensin Expression by Porphyromonas gingivalis-Infected Human Gingival Graft Transplanted in nu/nu Mouse Subdermis.
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DOI：
10.1267/ahc.12033
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发表时间：
2013-02-28
影响因子：
2.4
通讯作者：
Tsukinoki K
中科院分区：
文献类型：
--
作者：
To M;Kamata Y;Saruta J;Shimizu T;Sato T;Kondo Y;Hayashi T;Hamada N;Tsukinoki K
从细菌感染和宿主因素方面了解牙周病的发病是很重要的。宿主-细菌的相互作用可以在人类培养细胞和动物模型中引起，但这些模型只能提供有限的关于人类宿主对细菌攻击反应的生物学信息。需要利用人类牙龈组织开发体内模型。我们建立了nu/nu小鼠的体内模型，评估了人类牙龈细菌感染后的宿主防御。从牙周炎患者身上采集牙龈样本，移植到nu/nu小鼠真皮下。2周后，人类特异性标记物免疫组化反应阳性，证实了人类特征。我们使用这个模型来研究人类β-防御素-2 (hBD-2)，这是一种抗菌肽，有助于对细菌入侵的初始防御。利用实时聚合酶链反应、原位杂交和免疫组织化学技术，我们研究了hBD-2是否在人牙龈中被诱导表达，作为对牙龈卟啉单胞菌作为牙周病原体的反应。细菌感染2小时后，我们检测到hBD-2 mRNA的表达增加，该mRNA定位于人牙龈上皮。通过我们的体内模型，我们得出结论，hBD-2的增加可能在人类牙龈上皮早期防御细菌感染中发挥重要作用。
It is important to understand the onset of periodontal disease in terms of bacterial infection and host factors. Host-bacteria interactions can be elicited in human cultured cells and animal models, but these models provide only limited biological information about human host reactions against bacterial attacks. Development of an in vivo model using human gingival tissue is needed. We established an in vivo model using nu/nu mice and evaluated host defense following bacterial infection in human gingiva. Human gingival samples were collected from periodontitis patients and transplanted in nu/nu mouse subdermis. After 2 weeks, human characteristics were confirmed by positive immunohistochemical reactions for human-specific markers. We used this model to investigate human β-defensin-2 (hBD-2), an antimicrobial peptide that contributes to initial defense against bacterial invasion. Using real-time polymerase chain reaction, in situ hybridization, and immunohistochemistry, we investigated whether hBD-2 expression was induced in human gingiva as a response to Porphyromonas gingivalis as a periodontal pathogen. Two hours after infection with bacteria, we detected increased expression of hBD-2 mRNA, which was localized in the epithelium of human gingiva. Using our in vivo model, we concluded that increased hBD-2 may play an important role in early defense from bacterial infection in human gingival epithelium.
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影响因子：
2.4
作者：
Hioki, K;Kuramochi, T;Ito, M
通讯作者：
Ito, M
影响因子：
3.1
作者：
Krisanaprakornkit, S;Kimball, JR;Dale, DA
通讯作者：
Dale, DA
影响因子：
3.3
作者：
Bissell, J;Joly, S;Guthmiller, JM
通讯作者：
Guthmiller, JM
影响因子：
4.4
作者：
Pivarcsi, A;Bodai, L;Kemény, L
通讯作者：
Kemény, L
影响因子：
3.5
作者：
Mahanonda, R.;Sa-Ard-Iam, N.;Pichyangkul, S.
通讯作者：
Pichyangkul, S.