Precise levels of the Drosophila adaptor protein Dreadlocks maintain the size and stability of germline ring canals.
Precise levels of the Drosophila adaptor protein Dreadlocks maintain the size and stability of germline ring canals.
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果蝇衔接蛋白Dreadlocks的精确水平维持生殖系环管的大小和稳定性。
DOI：
10.1242/jcs.254730
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发表时间：
2021-04-15
影响因子：
4
通讯作者：
Lewellyn L
中科院分区：
文献类型：
--
作者：
Stark K;Crowe O;Lewellyn L
细胞间的桥对于许多生物体的生育力是必不可少的。发育中的果蝇卵已成为研究细胞间桥的首要模型系统。在卵子发生过程中，卵母细胞通过相对较大的细胞间桥或环管与支持细胞相连。一旦形成，环形通道的直径增加了20倍，以支持物质从滋养细胞向卵母细胞的移动。在这里，我们证明了一个新的作用，保守的SH 2/SH 3衔接蛋白Dreadlocks（码头）在调节环管的大小和结构稳定性的种系。在整个卵子发生过程中，Dock定位于生殖系环管。Dock的缺失导致环管直径的显著减小，并且Dock的过表达导致环管结构的显著缺陷和滋养细胞多核化。Dock的SH 2结构域是Src 64（也称为Src 64 B）下游的环管定位所必需的，并且一个或多个SH 3结构域的功能是强过表达表型所必需的。遗传相互作用和定位研究表明，码头促进WASP介导的Arp 2/3激活，以确定环管的大小和调节生长。总结：Drosophila Dock可能在WASp和Arp 2/3复合物的下游发挥作用，以调节发育卵室中生殖系环管的大小和稳定性。
Intercellular bridges are essential for fertility in many organisms. The developing fruit fly egg has become the premier model system to study intercellular bridges. During oogenesis, the oocyte is connected to supporting nurse cells by relatively large intercellular bridges, or ring canals. Once formed, the ring canals undergo a 20-fold increase in diameter to support the movement of materials from the nurse cells to the oocyte. Here, we demonstrate a novel role for the conserved SH2/SH3 adaptor protein Dreadlocks (Dock) in regulating ring canal size and structural stability in the germline. Dock localizes at germline ring canals throughout oogenesis. Loss of Dock leads to a significant reduction in ring canal diameter, and overexpression of Dock causes dramatic defects in ring canal structure and nurse cell multinucleation. The SH2 domain of Dock is required for ring canal localization downstream of Src64 (also known as Src64B), and the function of one or more of the SH3 domains is necessary for the strong overexpression phenotype. Genetic interaction and localization studies suggest that Dock promotes WASp-mediated Arp2/3 activation in order to determine ring canal size and regulate growth. Summary: Drosophila Dock likely functions downstream of WASp and the Arp2/3 complex to regulate the size and stability of the germline ring canals in the developing egg chamber.
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DOI：
10.4161/bioa.25744
发表时间：
2013-05
期刊：
Bioarchitecture
影响因子：
--
作者：
Chaki SP;Rivera GM
通讯作者：
Rivera GM
影响因子：
64.5
作者：
Hing, H;Xiao, J;Zipursky, SL
通讯作者：
Zipursky, SL
影响因子：
16.2
作者：
Fan, XP;Labrador, JP;Bashaw, GJ
通讯作者：
Bashaw, GJ
影响因子：
4.6
作者：
Bogard, Nicholas;Lan, Lan;Cohen, Robert S.
通讯作者：
Cohen, Robert S.
影响因子：
4.8
作者：
Hudson, Andrew M.;Cooley, Lynn
通讯作者：
Cooley, Lynn