Identification of CD157-Positive Vascular Endothelial Stem Cells in Mouse Retinal and Choroidal Vessels: Fluorescence-Activated Cell Sorting Analysis.
Identification of CD157-Positive Vascular Endothelial Stem Cells in Mouse Retinal and Choroidal Vessels: Fluorescence-Activated Cell Sorting Analysis.
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DOI：
10.1167/iovs.63.4.5
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发表时间：
2022-04-01
影响因子：
4.4
通讯作者：
Takakura N
中科院分区：
文献类型：
--
作者：
Wakabayashi T;Naito H;Iba T;Nishida K;Takakura N
CD157（也称为Bst 1）阳性血管内皮干细胞（VESC），有助于血管再生，最近已在小鼠器官中发现，包括视网膜，脑，肝，肺，心脏和皮肤。然而，VESC尚未在脉络膜中被鉴定。本研究的目的是鉴定脉络膜血管中的VESC，并建立分离视网膜和脉络膜VESC的方案。我们建立了一个有效的协议，以创建单细胞悬浮液从新鲜分离的小鼠视网膜和脉络膜的酶消化使用分散酶，胶原酶和II型胶原酶。使用荧光激活细胞分选术（FACS）分选CD157阳性VESC（定义为CD31 + CD45 − CD157+细胞）。在小鼠视网膜中，基于FACS分析，在CD31 + CD45 −内皮细胞（EC）中，1.6 ± 0.2%为CD157阳性VESC。在小鼠脉络膜中，在CD31 + CD45 − EC中，4.5 ± 0.4%是VESC。在血管内皮生长因子（VEGF）体外培养条件下，与CD157阴性的非VESCs相比，CD157阳性的VESCs产生了更多数量的EC网络。EC网络面积（定义为每个视野中CD31阳性面积占总面积的比率）分别为4.21 ± 0.39%（视网膜VESCs）和0.27 ± 0.12%（视网膜非VESCs）（P <0.01）。脉络膜VESCs和脉络膜非VESCs的EC网络面积分别为8.59 ± 0.78%和0.14 ± 0.04%（P <0.01）。VESC位于大血管中，但不在毛细血管中。我们证实了小鼠视网膜和脉络膜中CD157阳性VESCs的不同群体。VESC位于大血管中并具有增殖潜力。目前的研究结果可能为眼血管疾病的研究和治疗开辟新的途径。
CD157 (also known as Bst1) positive vascular endothelial stem cells (VESCs), which contribute to vascular regeneration, have been recently identified in mouse organs, including the retinas, brain, liver, lungs, heart, and skin. However, VESCs have not been identified in the choroid. The purpose of this study was to identify VESCs in choroidal vessels and to establish the protocol to isolate retinal and choroidal VESCs. We established an efficient protocol to create single-cell suspensions from freshly isolated mouse retina and choroid by enzymatic digestion using dispase, collagenase, and type II collagenase. CD157-positive VESCs, defined as CD31+CD45−CD157+ cells, were sorted using fluorescence-activated cell sorting (FACS). In mouse retina, among CD31+CD45− endothelial cells (ECs), 1.6 ± 0.2% were CD157-positive VESCs, based on FACS analysis. In mouse choroid, among CD31+CD45− ECs, 4.5 ± 0.4% were VESCs. The CD157-positive VESCs generated a higher number of EC networks compared with CD157-negative non-VESCs under vascular endothelial growth factor (VEGF) in vitro cultures. The EC network area, defined as the ratio of the CD31-positive area to the total area in each field, was 4.21 ± 0.39% (retinal VESCs) and 0.27 ± 0.12% (retinal non-VESCs), respectively (P < 0.01). The EC network area was 8.59 ± 0.78% (choroidal VESCs) and 0.14 ± 0.04% (choroidal non-VESCs), respectively (P < 0.01). The VESCs were located in large blood vessels but not in the capillaries. We confirmed distinct populations of CD157-positive VESCs in both mouse retina and choroid. VESCs are located in large vessels and have the proliferative potential. The current results may open new avenues for the research and treatment of ocular vascular diseases.
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影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
DOI：
10.1111/j.1749-6632.1982.tb25720.x
发表时间：
1982-01-01
影响因子：
5.2
作者：
FOLKMAN, J
通讯作者：
FOLKMAN, J
影响因子：
16.6
作者：
Benedicto I;Lehmann GL;Ginsberg M;Nolan DJ;Bareja R;Elemento O;Salfati Z;Alam NM;Prusky GT;Llanos P;Rabbany SY;Maminishkis A;Miller SS;Rafii S;Rodriguez-Boulan E
通讯作者：
Rodriguez-Boulan E
影响因子：
3.5
作者：
HIRATA, Y;KIMURA, N;HIRANO, T
通讯作者：
HIRANO, T
影响因子：
32.4
作者：
Mondor, Isabelle;Jorquera, Audrey;Bajenoff, Marc
通讯作者：
Bajenoff, Marc